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  4. Recombinant Clostridium kluyveri NAD-dependent 4-hydroxybutyrate dehydrogenase (4hbD)

Recombinant Clostridium kluyveri NAD-dependent 4-hydroxybutyrate dehydrogenase (4hbD)

  • 中文名稱：
    Recombinant Clostridium kluyveri NAD-dependent 4-hydroxybutyrate dehydrogenase(4hbD)
  • 貨號(hào)：
    CSB-YP327911CWX
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Clostridium kluyveri NAD-dependent 4-hydroxybutyrate dehydrogenase(4hbD)
  • 貨號(hào)：
    CSB-EP327911CWX-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Clostridium kluyveri NAD-dependent 4-hydroxybutyrate dehydrogenase(4hbD)
  • 貨號(hào)：
    CSB-BP327911CWX
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Clostridium kluyveri NAD-dependent 4-hydroxybutyrate dehydrogenase(4hbD)
  • 貨號(hào)：
    CSB-MP327911CWX
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    4hbD
  • Uniprot No.：
  • 別名：
    4hbD; CKL_3014NAD-dependent 4-hydroxybutyrate dehydrogenase; 4HBD; EC 1.1.1.61
  • 種屬：
    Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-371
  • 氨基酸序列
    MKLLKLAPDV YKFDTAEEFM KYFKVGKGDF ILTNEFLYKP FLEKFNDGAD AVFQEKYGLG EPSDEMINNI IKDIGDKQYN RIIAVGGGSV IDIAKILSLK YTDDSLDLFE GKVPLVKNKE LIIVPTTCGT GSEVTNVSVA ELKRRHTKKG IASDELYATY AVLVPEFIKG LPYKFFVTSS VDALIHATEA YVSPNANPYT DMFSVKAMEL ILNGYMQMVE KGNDYRVEII EDFVIGSNYA GIAFGNAGVG AVHALSYPIG GNYHVPHGEA NYLFFTEIFK TYYEKNPNGK IKDVNKLLAG ILKCDESEAY DSLSQLLDKL LSRKPLREYG MKEEEIETFA DSVIEGQQRL LVNNYEPFSR EDIVNTYKKL Y
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

問答及客戶評(píng)論

■ 常見問題解答
Q:

Could you please quote this 4-hydroxybutyrate dehydrogenase in all expression systems?
I also request for the enzyme activity (unit) for this enzyme.
https://www.cusabio.com/index.php?m=search&c=index&a=init&dosubmit=1&catid=init&typeid=&siteid=1&q=4-hydroxybutyrate+dehydrogenase

A:
Thanks for your interest in our product. Our distributor will send you the price soon, and pls check the detail information as follows:
Recombinant Clostridium kluyveri NAD-dependent 4-hydroxybutyrate dehydrogenase(4hbD)
CSB-YP327911CWX >> Yeast
CSB-EP327911CWX >> E.coli
CSB-BP327911CWX >> Baculovirus
CSB-MP327911CWX >> Mammalian cell
Expression region: 1-371aa; Full length.
Tag Information: EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged; YP: N-terminal 6xHis-tagged
Sequenece:

MKLLKLAPDVYKFDTAEEFMKYFKVGKGDFILTNEFLYKPFLEKFNDGADAVFQEKYGLGEPSDEMINNIIKDIGDKQYNRIIAVGGGSVIDIAKILSLKYTDDSLDLFEGKVPLVKNKELIIVPTTCGTGSEVTNVSVAELKRRHTKKGIASDELYATYAVLVPEFIKGLPYKFFVTSSVDALIHATEAYVSPNANPYTDMFSVKAMELILNGYMQMVEKGNDYRVEIIEDFVIGSNYAGIAFGNAGVGAVHALSYPIGGNYHVPHGEANYLFFTEIFKTYYEKNPNGKIKDVNKLLAGILKCDESEAYDSLSQLLDKLLSRKPLREYGMKEEEIETFADSVIEGQQRLLVNNYEPFSREDIVNTYKKLY


Sorry, as we haven't tested the activity, I'm afraid we can't guanrantee the activity of this enzyme.
However, as what we provide is the full length protein, and it's purified under very mild condition, so in theory, it should be active.
We recommend you to purchase a small size to have try. If you have any question during the use of this product, pls feel free to contact us.


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