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  4. Recombinant Chilobrachys jingzhao Delta-theraphotoxin-Cj1a (JZTX-I)

Recombinant Chilobrachys jingzhao Delta-theraphotoxin-Cj1a (JZTX-I)

  • 中文名稱：
    Recombinant Chilobrachys jingzhao Delta-theraphotoxin-Cj1a(JZTX-I)
  • 貨號：
    CSB-YP306704DRU
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Chilobrachys jingzhao Delta-theraphotoxin-Cj1a(JZTX-I)
  • 貨號：
    CSB-EP306704DRU
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Chilobrachys jingzhao Delta-theraphotoxin-Cj1a(JZTX-I)
  • 貨號：
    CSB-EP306704DRU-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Chilobrachys jingzhao Delta-theraphotoxin-Cj1a(JZTX-I)
  • 貨號：
    CSB-BP306704DRU
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Chilobrachys jingzhao Delta-theraphotoxin-Cj1a(JZTX-I)
  • 貨號：
    CSB-MP306704DRU
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    N/A
  • Uniprot No.：
  • 別名：
    Delta-theraphotoxin-Cg1a 1; Delta-TRTX-Cg1a; Jingzhaotoxin-1; Jingzhaotoxin-I; JZTX-I; Peptide F5-24.92
  • 種屬：
    Chilobrachys jingzhao (Chinese earth tiger tarantula) (Chilobrachys guangxiensis)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    30-62
  • 氨基酸序列
    A CGQFWWKCGE GKPPCCANFA CKIGLYLCIW SP
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Inhibits voltage-gated sodium channels, preferentially subtype Nav1.5/SCN5A (in cardiac myocytes), but also Nav1.6/SCN8A and Nav1.7/SCN9A (TTX-sensitive Nav in rat DRG neurons) and invertebrate Nav (in insect neurons) as well as voltage-gated potassium channels of the subtype Kv2.1/KCNB1. Is suggested to bind to site 3 of the sodium channels and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. On potassium channels, inhibits activation of channels with an IC(50) of 8.05 uM through a voltage sensor-trapping mechanism. Increases muscle contraction in several assays (mouse phrenic nerve-diaphragm, toad heart, rat vas deferens) and is suggested to act both presynaptically and postsynaptically.; Moderately inhibits voltage-gated sodium channels and weakly inhibits voltage-gated potassium channel. Inhibits the inactivation of rat Nav1.2/SCN2A (IC(50)=870 nM), rat Nav1.3/SCN3A (IC(50)=845 nM), rat Nav1.4/SCN4A (IC(50)=339 nM), human Nav1.5/SCN5A (IC(50)=335 nM) and human Nav1.7/SCN9A sodium channels (IC(50)=348 nM). The toxin delays the inactivation of sodium channels without affecting the activation and steady-state inactivation kinetics in the physiological range of voltages. Site-directed mutagenesis of the sodium channel indicates that the toxin interacts with site 3 located at the extracellular S3-S4 linker of domain IV. On potassium channels, it inhibits activation of channels with an IC(50) of 8.05 uM through a voltage sensor-trapping mechanism. It increases muscle contraction in several assays (mouse phrenic nerve-diaphragm, toad heart, rat vas deferens) and is suggested to act both presynaptically and postsynaptically.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Huwentoxin-1 family, Jztx-1 subfamily
  • 組織特異性：
    Expressed by the venom gland.


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