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Recombinant Cat Major allergen I polypeptide chain 2 (CH2)

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  • 中文名稱:
    Recombinant Cat Major allergen I polypeptide chain 2 (CH2)
  • 品名簡稱:
    Recombinant Cat CH2 protein
  • 貨號(hào):
    CSB-EP326973CA
  • 說明書:
  • 規(guī)格:
    ¥1836
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產(chǎn)品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 生物活性:
    Not Test
  • 基因名:
    CH2
  • Uniprot No.:
  • 種屬:
    Felis catus (Cat) (Felis silvestris catus)
  • 蛋白長度:
    Full Length of Mature Protein
  • 來源:
    E.coli
  • 分子量:
    17.0 kDa
  • 表達(dá)區(qū)域:
    18-109aa
  • 氨基酸序列
    VKMAETCPIFYDVFFAVANGNELLLDLSLTKVNATEPERTAMKKIQDCYVENGLISRVLDGLVMTTISSSKDCMGEAVQNTVEDLKLNTLGR
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽:
    C-terminal 6xHis-tagged
  • 產(chǎn)品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 復(fù)溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項(xiàng):
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 基因功能參考文獻(xiàn):
    1. Cat Fel d 1 is an ABP-like molecule in which monomeric chains 1 and 2 are the equivalent of the ABPA and ABPBG monomers, respectively. These findings suggest that the biological and molecular function of Fel d 1 is similar to that of ABP in chemical communication, possibly via pheromone and/or steroid binding. PMID: 29771985
    2. The newly identified allergenic epitope of the Fel d 1 is useful in a design of the CRIT and CRD for cat allergy. PMID: 26797272
    3. Conformational effects brought upon by glycosylation were identified, potentially involved in cavity volume regulation. Only the central Ca2+ ion remains coordinated to Fel d 1 in biological solutions, impairing its role in modulating phospholipase A2. PMID: 26134118
    4. Fel d 1 enhances signaling through the innate receptors TLR4 & TLR2. It does not bind the TLR4/MD2 complex but it does bind the TLR4 agonist lipopolysaccharide. A lipid transfer mechanism may be involved in Fel d 1 TLR signaling enhancement. PMID: 23878318
    5. Truncated dimers of Fel d 1 were identified, probably resulting from proteolytic degradation of both chains and present in all cats. Core fragments are largely distributed among anatomical sites of production and especially well represented in anal sac. PMID: 20145408
    6. Crystals of Fel d 1 (1+2) were obtained using the hanging-drop vapour-diffusion method. PMID: 16511003
    7. Fel d 1 could provoke an allergic response through the modulation of phospholipase A2 PMID: 17543334
    8. The study identified an immunodominant region of Fel d 1 which is frequently recognized by CD4(+) T cells from atopic individuals and contains epitopes that are restricted by very common HLA alleles. PMID: 19489916

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  • 亞細(xì)胞定位:
    Secreted.
  • 蛋白家族:
    Secretoglobin family
  • 組織特異性:
    The long form is preferentially expressed in the salivary gland, while the short form is preferentially expressed in the skin.


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