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Recombinant Calloselasma rhodostoma Snaclec rhodocytin subunit alpha

In Stock
  • 中文名稱:
    Recombinant Calloselasma rhodostoma Snaclec rhodocytin subunit alpha
  • 貨號(hào):
    CSB-EP888242CBG
  • 規(guī)格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

產(chǎn)品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    N/A
  • Uniprot No.:
  • 別名:
    ; Snaclec rhodocytin subunit alpha; Aggretin alpha chain; Rhodoaggretin subunit alpha
  • 種屬:
    Calloselasma rhodostoma (Malayan pit viper) (Agkistrodon rhodostoma)
  • 蛋白長度:
    Full Length
  • 來源:
    E.coli
  • 分子量:
    31.8kDa
  • 表達(dá)區(qū)域:
    1-136aa
  • 氨基酸序列
    GLEDCDFGWSPYDQHCYQAFNEQKTWDEAEKFCRAQENGAHLASIESNGEADFVSWLISQKDELADEDYVWIGLRAQNKEQQCSSEWSDGSSVSYENLIDLHTKKCGALEKLTGFRKWVNYYCEQMHAFVCKLLPY
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽:
    N-terminal 6xHis-SUMO-tagged
  • 產(chǎn)品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲(chǔ)存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項(xiàng):
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述:

    The full-length cDNA ORF of Calloselasma rhodostoma Snaclec rhodocytin subunit alpha was N-terminally tagged a 6xHis-SUMO and then expressed in the E.coli cells. The recombinant Calloselasma rhodostoma Snaclec rhodocytin subunit alpha thus obtained was purified using SDS-PAGE analysis and got over 90% of purity. Under reducing conditions, this protein migrated to an approximately 28 kDa molecular mass band on the SDS-PAGE gel. This recombinant Snaclec rhodocytin subunit alpha protein may be used to produce specific antibodies or in the studies of snaclec rhodocytin-mediated signal transduction.

    Snaclec rhodocytin is a disulfide‐linked heterodimeric C‐type lectin derived from Calloselasma rhodostoma and is made up of α‐subunits and β‐subunits. The interaction between snaclec rhodocytin and C‐type lectin‐like receptor 2 (CLEC-2) induces platelet aggregation. CLEC‐2 physiologically binding to podoplanin (PDPN), which is expressed on some tumor cell types, participates in tumor cell‐induced platelet aggregation and tumor metastasis. This may propose a possible source of anti‐CLEC‐2 drugs for both antiplatelet and antimetastasis therapy through rhodocytin modification.

  • Datasheet & COA:
    Please contact us to get it.

引用文獻(xiàn)

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Elicits platelet aggregation by the binding to the C-type lectin domain family 1 member B (CLEC1B/CLEC2). Binding leads to tyrosine phosphorylation in the cytoplasmic tail of CLEC1B, which promotes the binding of spleen tyrosine kinase (Syk), subsequent activation of PLC-gamma-2, and platelet activation and aggregation. Binding to GPIbalpha (GP1BA) and alpha-2/beta-1 (ITGA2/ITGB1) may also induce aggregation, but this is controversial.
  • 亞細(xì)胞定位:
    Secreted.
  • 蛋白家族:
    Snaclec family
  • 組織特異性:
    Expressed by the venom gland.


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