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  4. Recombinant Burkholderia xenovorans 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (bphD)

Recombinant Burkholderia xenovorans 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (bphD)

  • 中文名稱：
    Recombinant Burkholderia xenovorans 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase(bphD)
  • 貨號：
    CSB-YP342870BNV
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Burkholderia xenovorans 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase(bphD)
  • 貨號：
    CSB-EP342870BNV
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Burkholderia xenovorans 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase(bphD)
  • 貨號：
    CSB-EP342870BNV-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Burkholderia xenovorans 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase(bphD)
  • 貨號：
    CSB-BP342870BNV
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Burkholderia xenovorans 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase(bphD)
  • 貨號：
    CSB-MP342870BNV
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    bphD
  • Uniprot No.：
  • 別名：
    bphD; Bxeno_C1120; Bxe_C11862-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase; HOPDA hydrolase; EC 3.7.1.8; 2,6-dioxo-6-phenylhexa-3-enoate hydrolase
  • 種屬：
    Burkholderia xenovorans (strain LB400)
  • 蛋白長度：
    full length protein
  • 表達區(qū)域：
    1-286
  • 氨基酸序列
    MTALTESSTS KFVKINEKGF SDFNIHYNEA GNGETVIMLH GGGPGAGGWS NYYRNVGPFV DAGYRVILKD SPGFNKSDAV VMDEQRGLVN ARAVKGLMDA LDIDRAHLVG NSMGGATALN FALEYPDRIG KLILMGPGGL GPSMFAPMPM EGIKLLFKLY AEPSYETLKQ MLQVFLYDQS LITEELLQGR WEAIQRQPEH LKNFLISAQK APLSTWDVTA RLGEIKAKTF ITWGRDDRFV PLDHGLKLLW NIDDARLHVF SKCGHWAQWE HADEFNRLVI DFLRHA
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Catalyzes an unusual C-C bond hydrolysis of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) to produce benzoic acid and 2-hydroxy-2,4-pentadienoic acid (HPD).
  • 基因功能參考文獻：
    1. Data indicate that the chlorine substituent is accommodated by a hydrophobic pocket that is larger than the homologous site in BphDLB400 from Burkholderia xenovorans LB400. PMID: 23879719
    2. Site-directed single mutants of BphD in which catalytic residues His-265 and Ser-112 were replaced with Ala possessed 10(4)-fold reduced k(cat) values, and in each case, the C-C cleavage step was rate-limiting. PMID: 17029401
    3. replacements with Gln and Lys for Arg-190 of BphD led to 400- and 700-fold decreases, respectively, in k(cat) PMID: 17029402
  • 蛋白家族：
    AB hydrolase superfamily, BphD family
  • 數(shù)據(jù)庫鏈接：


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