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Recombinant Bovine Tropomyosin alpha-1 chain (TPM1)

  • 中文名稱:
    牛TPM1重組蛋白
  • 貨號:
    CSB-YP702512BO
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    牛TPM1重組蛋白
  • 貨號:
    CSB-EP702512BO
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    牛TPM1重組蛋白
  • 貨號:
    CSB-EP702512BO-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    牛TPM1重組蛋白
  • 貨號:
    CSB-BP702512BO
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    牛TPM1重組蛋白
  • 貨號:
    CSB-MP702512BO
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
  • Uniprot No.:
  • 別名:
    TPM1; Tropomyosin alpha-1 chain; Alpha-tropomyosin; Tropomyosin-1
  • 種屬:
    Bos taurus (Bovine)
  • 蛋白長度:
    full length protein
  • 表達區域:
    1-284
  • 氨基酸序列
    MDAIKKKMQM LKLDKENALD RAEQAEADKK AAEDRSKQLE DELVSLQKKL KATEDELDKY SEALKDAQEK LELAEKKATD AEADVASLNR RIQLVEEELD RAQERLATAL QKLEEAEKAA DESERGMKVI ESRAQKDEEK MEIQEIQLKE AKHIAEDADR KYEEVARKLV IIESDLERAE ERAELSEGKC AELEEELKTV TNNLKSLEAQ AEKYSQKEDK YEEEIKVLSD KLKEAETRAE FAERSVTKLE KSIDDLEDEL YAQKLKYKAI SEELDHALND MTSI
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
  • 基因功能參考文獻:
    1. The first step of hypertrophic cardiomyopathy pathogenesis with E99K is increased calcium sensitivity and decreased calcium cooperativity, which result in larger tension during partial activation to cause a diastolic problem. PMID: 25451174
    2. over-inhibition of the actomyosin interaction by E40K and E54K Tm mutants leads to a decreased force-generating ability at systole, which is the main mechanism underlying the early pathogenesis of DCM. PMID: 23077624
    3. The tendency of smooth muscle tropomyosin to form semi-rigid polymers with continuous and undampened rigidity may compensate for the lack of troponin-based structural support in smooth muscles. PMID: 20682264
    4. Deletion of regions 2-3 in tropomyosin alpha resulted in an 60 % decrease in both isometric tension and stiffness of tropomyosin-reconstituted myocardium. PMID: 14500764
    5. Tropomyosin is primarily responsible for the change in the kinetic constants of the elementary steps of the cross bridge cycle. PMID: 14742733
    6. Tm affects the conformation of actin so as to increase the area of hydrophobic interaction between actin and myosin molecules PMID: 16980359

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  • 亞細胞定位:
    Cytoplasm, cytoskeleton.
  • 蛋白家族:
    Tropomyosin family
  • 數據庫鏈接:


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