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  4. Recombinant Bovine Recoverin (RCVRN)

Recombinant Bovine Recoverin (RCVRN)

  • 中文名稱：
    牛RCVRN重組蛋白
  • 貨號：
    CSB-YP019519BO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    牛RCVRN重組蛋白
  • 貨號：
    CSB-EP019519BO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    牛RCVRN重組蛋白
  • 貨號：
    CSB-EP019519BO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    牛RCVRN重組蛋白
  • 貨號：
    CSB-BP019519BO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    牛RCVRN重組蛋白
  • 貨號：
    CSB-MP019519BO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RCVRN
  • Uniprot No.：
  • 別名：
    RCVRN; RCV1; Recoverin; p26
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-202
  • 氨基酸序列
    GNSKSGALS KEILEELQLN TKFTEEELSS WYQSFLKECP SGRITRQEFQ TIYSKFFPEA DPKAYAQHVF RSFDANSDGT LDFKEYVIAL HMTSAGKTNQ KLEWAFSLYD VDGNGTISKN EVLEIVTAIF KMISPEDTKH LPEDENTPEK RAEKIWGFFG KKDDDKLTEK EFIEGTLANK EILRLIQFEP QKVKEKLKEK KL
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Acts as a calcium sensor and regulates phototransduction of cone and rod photoreceptor cells. Modulates light sensitivity of cone photoreceptor in dark and dim conditions. In response to high Ca(2+) levels induced by low light levels, prolongs RHO/rhodopsin activation in rod photoreceptor cells by binding to and inhibiting GRK1-mediated phosphorylation of RHO/rhodopsin. Plays a role in scotopic vision/enhances vision in dim light by enhancing signal transfer between rod photoreceptors and rod bipolar cells. Improves rod photoreceptor sensitivity in dim light and mediates response of rod photoreceptors to facilitate detection of change and motion in bright light.
  • 基因功能參考文獻：
    1. A novel rhodopsin-kinase-binding site within the C-terminal region of recoverin. PMID: 21299498
    2. (15) N NMR relaxation data suggest that Ca(2+)-free recoverin undergoes millisecond conformational dynamics at particular amide sites throughout the protein. PMID: 21465563
    3. Ca2+-myristoyl switch in the neuronal calcium sensor requires different functions of Ca2+-binding sites PMID: 12393897
    4. Recoverin is a zinc-binding protein PMID: 12643543
    5. Myristoylation changes the calcium dependent conformation of this protein. PMID: 12686556
    6. Recoverin and rhodopsin kinase have roles in a Ca2+-dependent feedback loop in membrane rafts from rod outer segments PMID: 15355976
    7. an interaction surface for the recoverin target rhodopsin kinase is constituted upon Ca2+ binding to the non-acylated mutant PMID: 15843174
    8. Recoverin binds exclusively to an amphipathic peptide at the N terminus of rhodopsin kinase PMID: 16675451
    9. Ca2+/recoverin has a role in regulating rhodopsin mutants associated with retinitis pigmentosa PMID: 16934219
    10. propose a novel role of the C-terminal segment of recoverin as an internal modulator of Ca(2+) sensitivity PMID: 17015448
    11. Time-resolved fluorescence shows biexponential fluorescence decay. The slower time constant was affected by Ca2+-induced conformational changes in WT & RecE85Q (disabled EF-hand 2), & not RecE121Q (EF-hand 3 nonfunctional) with a clamped myristoyl group. PMID: 17078090
    12. Properties of the charged carboxy-terminus are consistent with a role of this region as an internal effector region that prolongs the time recoverin stays on the membrane by influencing its Ca2+-sensitivity PMID: 18034895

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  • 亞細胞定位：
    Photoreceptor inner segment. Cell projection, cilium, photoreceptor outer segment. Photoreceptor outer segment membrane; Lipid-anchor; Cytoplasmic side. Perikaryon.
  • 蛋白家族：
    Recoverin family
  • 組織特異性：
    Expressed in the retina (at protein level). Expressed in the pineal gland (at protein level).
  • 數據庫鏈接：


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