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  2. 蛋白
  3. 重組蛋白
  4. Recombinant Bovine Phospholipase A2 (PLA2G1B)

Recombinant Bovine Phospholipase A2 (PLA2G1B)

  • 中文名稱：
    牛PLA2G1B重組蛋白
  • 貨號(hào)：
    CSB-YP018090BO
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    牛PLA2G1B重組蛋白
  • 貨號(hào)：
    CSB-EP018090BO
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    牛PLA2G1B重組蛋白
  • 貨號(hào)：
    CSB-EP018090BO-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    牛PLA2G1B重組蛋白
  • 貨號(hào)：
    CSB-BP018090BO
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    牛PLA2G1B重組蛋白
  • 貨號(hào)：
    CSB-MP018090BO
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    PLA2G1B; Phospholipase A2; EC 3.1.1.4; Group IB phospholipase A2; Phosphatidylcholine 2-acylhydrolase 1B
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長(zhǎng)度：
    Full Length of Mature Protein
  • 表達(dá)區(qū)域：
    23-145
  • 氨基酸序列
    ALWQFNGM IKCKIPSSEP LLDFNNYGCY CGLGGSGTPV DDLDRCCQTH DNCYKQAKKL DSCKVLVDNP YTNNYSYSCS NNEITCSSEN NACEAFICNC DRNAAICFSK VPYNKEHKNL DKKNC
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Secretory calcium-dependent phospholipase A2 that primarily targets dietary phospholipids in the intestinal tract. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. May play a role in the biosynthesis of N-acyl ethanolamines that regulate energy metabolism and inflammation in the intestinal tract. Hydrolyzes N-acyl phosphatidylethanolamines to N-acyl lysophosphatidylethanolamines, which are further cleaved by a lysophospholipase D to release N-acyl ethanolamines. May act in an autocrine and paracrine manner. Has anti-helminth activity in a process regulated by gut microbiota. Upon helminth infection of intestinal epithelia, directly affects phosphatidylethanolamine contents in the membrane of helminth larvae, likely controlling an array of phospholipid-mediated cellular processes such as membrane fusion and cell division while providing for better immune recognition, ultimately reducing larvae integrity and infectivity.
  • 基因功能參考文獻(xiàn)：
    1. Phospholipase A2 isoforms are differentially regulated; they selectively participate in retinal signaling in an experimental model of age-related macular degeneration. PMID: 22732705
    2. results suggest the hydrophobic surfactant protein, SP-B, protects alveolar surfactant phospholiopids from hydrolysis mediated by multiple sPLA(2) in both vesicles (alveolar subphase) and monomolecular films (air-liquid interface) PMID: 22037357
    3. crystal structure of the triple mutant pancreatic phospholipase A2 with calcium. PMID: 12460575
    4. NMR characterization of a unique low-barrier hydrogen bond between an active site residue from the enzyme and a bound inhibitor: the complex between secreted phospholipase A(2) (sPLA(2), from bovine pancreas) and a transition-state analog inhibitor HK32 PMID: 12798689
    5. The final protein model consists of 123 amino-acid residues, two calcium ions, one chloride ion, 243 water molecules and six 2-methyl-2,4-pentanediol molecules. PMID: 16508077
    6. The crystal structure of the triple mutant (K53,56,121M) of bovine pancreatic phospholipase A2 complexed with an organic molecule, p-methoxybenzoic acid (anisic acid), is reported. PMID: 16552140
    7. The quadruple mutant of phospholipase A2 has monoclinic or trigonal crystal structures and results suggested evidence for the involvement of the second calcium and surface loop in interfacial binding. PMID: 16790927

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  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Phospholipase A2 family
  • 數(shù)據(jù)庫(kù)鏈接：


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