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  4. Recombinant Bovine Osteopontin (SPP1)

Recombinant Bovine Osteopontin (SPP1)

  • 中文名稱：
    牛SPP1重組蛋白
  • 貨號：
    CSB-YP022603BO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    牛SPP1重組蛋白
  • 貨號：
    CSB-EP022603BO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    牛SPP1重組蛋白
  • 貨號：
    CSB-EP022603BO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    牛SPP1重組蛋白
  • 貨號：
    CSB-BP022603BO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    牛SPP1重組蛋白
  • 貨號：
    CSB-MP022603BO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    SPP1; OPN; Osteopontin; Bone sialoprotein 1; Secreted phosphoprotein 1; SPP-1
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    17-278
  • 氨基酸序列
    LPVK PTSSGSSEEK QLNNKYPDAV ATWLKPDPSQ KQTFLAPQNS VSSEETDDNK QNTLPSKSNE SPEQTDDLDD DDDNSQDVNS NDSDDAETTD DPDHSDESHH SDESDEVDFP TDIPTIAVFT PFIPTESAND GRGDSVAYGL KSRSKKFRRS NVQSPDATEE DFTSHIESEE MHDAPKKTSQ LTDHSKETNS SELSKELTPK AKDKNKHSNL IESQENSKLS QEFHSLEDKL DLDHKSEEDK HLKIRISHEL DSASSEVN
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Major non-collagenous bone protein that binds tightly to hydroxyapatite (Probable). Appears to form an integral part of the mineralized matrix (Probable). Probably important to cell-matrix interaction (Probable).; Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • 基因功能參考文獻：
    1. study first addressed in detail the LF/fOPN interaction and proposed a LF:fOPN 4/1 maximal stoichiometry. PMID: 28561851
    2. This implies that a majority of the acidic residues within OPN must be engaged in calcium interaction under physiological conditions. PMID: 24928493
    3. Genetic variations in the SPP1 promoter affect gene expression and the level of osteopontin secretion into bovine milk. PMID: 24961487
    4. Osteopontin, osteocalcin and OB-cadherin expression in Synthetic nanohydroxyapatite vs bovine hydroxyapatite cultured Osteoblastic-like cells. PMID: 25316140
    5. osteopontin (OPN) can serve as a process-directing agent for the intrafibrillar mineralization of collagen. PMID: 24140612
    6. Results suggest a mechanism of the interaction of UO2(2+) with bone metabolism and a new role for osteopontin (OPN) as a metal transporter. PMID: 24327307
    7. Upon induction of luteolysis, SPP1 serves as a signaling molecule to recruit or activate immune cells to facilitate luteal regression and tissue degradation. PMID: 24019509
    8. OPN strongly reduces the amount of biofilm formed in a well-defined laboratory model of acidogenic dental biofilm. PMID: 22879891
    9. This study demonstrates that adhesion of isolated neonatal rat osteoclasts in vitro was augmented on bovine milk osteopontin (bmOPN) with post-translational modifications (PTMs) compared to human Escherichia-coli-derived recombinant OPN without PTMs. PMID: 19854170
    10. topographical distribution of both the in vivo and in vitro phosphorylation sites of bone sialoprotein PMID: 15004024
    11. the bovine osteopontin gene has two functionally distinct clusters of haplotypes within the QTL region on chromosome 6 PMID: 15867146
    12. The relationship between polymorphisms in SPP1 and several factors affecting growth and development in beef cattle was studied. PMID: 17179534
    13. findings show significant association of osteoponitin variants with milk composition traits PMID: 17517737
    14. In this study, we have expressed bovine osteopontin in a prokaryotic system and identified the seven amino acid residues phosphorylated in vitro by CKII. PMID: 17615552
    15. The binding of bovine seminal proteins A1, A2, and 30 kDa and osteopontin to spermatozoa and the effects of oviductal fluid on protein binding are reported. PMID: 18207674
    16. Results of this study confirm for the first time, the expression of Opn in the ileum and ileocecal lymph node of cattle with paratuberculosis. PMID: 18620757
    17. Opn may be a key regulator against Mycobacterium avium ssp. paratuberculosis infection PMID: 18650284
    18. The role of OPN phosphate groups in adsorption to, incorporation into and inhibition of calcium oxalate monohydrate crystals was studied by comparing OPN isoforms differing in phosphorylation. PMID: 18703867
    19. SPP1 (also known as osteopontin) has a significant role in the modulation of milk protein gene expression. PMID: 19392827
    20. OPN may act as a carrier protein for LF in milk, and modulate the potent antimicrobial and immunostimulatory activities of the LF protein. PMID: 19477017
    21. the present study is the first to demonstrate functionally that SPP1 directly binds specific integrins to promote trophectoderm cell migration and attachment to luminal epithelium that may be critical to conceptus elongation and implantation. PMID: 19571258
    22. Polymorphisms in the genomic sequence of SPP1 can influence the number of somatic cells in milk and, potentially, influence susceptibility to mastitis. PMID: 19765294

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Osteopontin family
  • 組織特異性：
    Bone, inner ear, kidney, uterus, lung, brain, epidermis.
  • 數據庫鏈接：


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