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  4. Recombinant Bovine Nitric oxide synthase, inducible (NOS2), partial

Recombinant Bovine Nitric oxide synthase, inducible (NOS2), partial

  • 中文名稱：
    牛NOS2重組蛋白
  • 貨號：
    CSB-YP636699BO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    牛NOS2重組蛋白
  • 貨號：
    CSB-EP636699BO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    牛NOS2重組蛋白
  • 貨號：
    CSB-EP636699BO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    牛NOS2重組蛋白
  • 貨號：
    CSB-BP636699BO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    牛NOS2重組蛋白
  • 貨號：
    CSB-MP636699BO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    NOS2; Nitric oxide synthase; inducible; EC 1.14.13.39; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; NOSII; Peptidyl-cysteine S-nitrosylase NOS2
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of proinflammatory mediators such as IL6 and IL8.
  • 基因功能參考文獻：
    1. polymorphism of the NOS2 gene may contribute to the susceptibility of Holstein cattle to bovine tuberculosis PMID: 26468216
    2. Vitamin D receptor activation, and inducible nitric oxide synthase (NOS2), were strongly induced during Cooperia oncophora reinfection. Several canonical pathways associated with NOS2 were impacted. PMID: 21414188
    3. The expression, localization, and distribution of 3 nitric oxide synthase enzymes through the estrous cycle in bovien fallopian tubes are reported. PMID: 23410122
    4. These results provide evidence for a high prevalence of subclinical endometritis in repeat breeding cows as well as the involvement of TNFalpha and iNOS pathways in the regulation of this pathological condition. PMID: 24597304
    5. Differential cell-specific and spatiotemporal expression of the EDN1 system and NOS in the bovine utero-placental unit may be associated with regulation of vascular and cellular functions during pregnancy. PMID: 22921266
    6. All infected calves had an increased number of cells expressing iNOS, nitrotyrosine and manganese superoxide dismutase in the inflamed lung tissue PMID: 21334636
    7. iNOS is expressed in ruminant granulosa cells and is regulated by gonadotrophins and oestradiol PMID: 21256181
    8. Expression level of NOS2 mRNA in endometrial biopsies from cows with puerperal endometritis is high. Highest expression of is found in cows with clinical endometritis. PMID: 20414720
    9. data suggest that the transcriptional inducible NOS response to octacalcium phosphate crystals involved both the p38 and the JNK MAPK pathways, probably under the control of activator protein-1 PMID: 16207333
    10. The expression of iNOS and NRAMP1 in the lymph nodes, lungs, and tuberculous granulomas in 8 bovine tuberculosis cases is reported. PMID: 16966449
    11. identified a 3471bp transcript for iNOS, isolated from RNA from alveolar macrophages stimulated with the intracellular pathogen Mycobacterium bovis PMID: 17336395
    12. The results showed that the expression of TNF-alpha, iNOS, and IL-6 in alveolar macrophages was up-regulated by stimulation with the recombinant Mce4A protein of M. bovis; in contrast, expression of IL-12 was unaffected. PMID: 17530193
    13. fibronectin fragment induced iNOS and COX-2 expression is downregulated in chondrocyte/agarose constructs PMID: 18677626
    14. study investigated hypothesis that CD40 signalling is impaired in Mycobacterium avium subspecies paratuberculosis(MAP)-infected macrophages; propose that MAP interferes with gene expression of iNOS and IL-12p40 genes downstream of p38 PMID: 19022505
    15. Isoforms of NOS (eNOS and iNOS) are present in oocytes of cattle from early folliculogenesis up to maturation; in vitro maturation influences amount of mRNA and NOS activity. PMID: 19327921

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  • 亞細胞定位：
    Cytoplasm, cytosol.
  • 蛋白家族：
    NOS family
  • 數據庫鏈接：


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