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  4. Recombinant Bovine Neuronal acetylcholine receptor subunit alpha-7 (CHRNA7), partial

Recombinant Bovine Neuronal acetylcholine receptor subunit alpha-7 (CHRNA7), partial

  • 中文名稱：
    Recombinant Bovine Neuronal acetylcholine receptor subunit alpha-7(CHRNA7),partial
  • 貨號：
    CSB-YP005393BO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Bovine Neuronal acetylcholine receptor subunit alpha-7(CHRNA7),partial
  • 貨號：
    CSB-EP005393BO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Bovine Neuronal acetylcholine receptor subunit alpha-7(CHRNA7),partial
  • 貨號：
    CSB-EP005393BO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Bovine Neuronal acetylcholine receptor subunit alpha-7(CHRNA7),partial
  • 貨號：
    CSB-BP005393BO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Bovine Neuronal acetylcholine receptor subunit alpha-7(CHRNA7),partial
  • 貨號：
    CSB-MP005393BO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    CHRNA7Neuronal acetylcholine receptor subunit alpha-7
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
  • 基因功能參考文獻：
    1. We conclude that residues in the M2 segment and flanking regions contribute to the unusual properties of the CHRNA7 receptor PMID: 21790604
    2. Deletion of a small cytoplasmic fragment close to the fourth transmembrane segment of the nicotinic alpha7 receptor (Glu437 to Arg447) abolished membrane expression PMID: 21729697
    3. Data show that basic residues at positions 208 and 210 are necessary for the assembly of alpha7 receptors. PMID: 21420959
    4. Cultured bovine retinal pigment epitheliumcells expressed nicotinic receptors containing alpha7 subunit PMID: 20932358
    5. Loop 9 has a different role in the function of homomeric and heteromeric receptors. PMID: 20043866
    6. Loop 3 and its docking to the alpha-helix is an important requirement for receptor assembly of the alpha7 nicotinic receptor. PMID: 19840217
    7. Bovine chromaffin cells have an alpha3alpha7beta4 nicotinic receptor PMID: 12438113
    8. a network of interactions formed by residues Lys-46, Asp-128, Asp-135, Asp-266, and possibly others appears to link agonist binding to channel gating PMID: 15611071
    9. involvement of Lys145 in the early steps of channel activation by acetylcholine PMID: 16129734
    10. slowly desensitizing mutant alpha7L248T could be an interesting tool for the study of channel activation in alpha7 receptors PMID: 19063861

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  • 亞細胞定位：
    Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Ligand-gated ion channel (TC 1.A.9) family, Acetylcholine receptor (TC 1.A.9.1) subfamily, Alpha-7/CHRNA7 sub-subfamily
  • 組織特異性：
    At least in chromaffin cells.
  • 數據庫鏈接：

    KEGG: bta:282178

    STRING: 9913.ENSBTAP00000020942

    UniGene: Bt.88



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