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Recombinant Bovine NEDD8 (NEDD8)

  • 中文名稱:
    牛NEDD8重組蛋白
  • 貨號:
    CSB-YP015684BO
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    牛NEDD8重組蛋白
  • 貨號:
    CSB-EP015684BO
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    牛NEDD8重組蛋白
  • 貨號:
    CSB-EP015684BO-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    牛NEDD8重組蛋白
  • 貨號:
    CSB-BP015684BO
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    牛NEDD8重組蛋白
  • 貨號:
    CSB-MP015684BO
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    NEDD8
  • Uniprot No.:
  • 別名:
    NEDD8NEDD8; Neddylin; Ubiquitin-like protein Nedd8
  • 種屬:
    Bos taurus (Bovine)
  • 蛋白長度:
    Full Length of Mature Protein
  • 表達區域:
    1-76
  • 氨基酸序列
    MLIKVKTLTG KEIEIDIEPT DKVERIKERV EEKEGIPPQQ QRLIYSGKQM NDEKTAADYK ILGGSVLHLV LALRGG
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53. Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins. Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M.
  • 亞細胞定位:
    Nucleus.
  • 蛋白家族:
    Ubiquitin family
  • 數據庫鏈接:

    KEGG: bta:286796

    STRING: 9913.ENSBTAP00000003514

    UniGene: Bt.275



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