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  4. Recombinant Bovine Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)

Recombinant Bovine Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)

  • 中文名稱：
    牛GAPDH重組蛋白
  • 貨號：
    CSB-YP009232BO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    牛GAPDH重組蛋白
  • 貨號：
    CSB-EP009232BO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    牛GAPDH重組蛋白
  • 貨號：
    CSB-EP009232BO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    牛GAPDH重組蛋白
  • 貨號：
    CSB-BP009232BO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    牛GAPDH重組蛋白
  • 貨號：
    CSB-MP009232BO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    GAPDH; GAPDGlyceraldehyde-3-phosphate dehydrogenase; GAPDH; EC 1.2.1.12; Peptidyl-cysteine S-nitrosylase GAPDH; EC 2.6.99.-
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-333
  • 氨基酸序列
    VKVGVNGFG RIGRLVTRAA FNSGKVDIVA INDPFIDLHY MVYMFQYDST HGKFNGTVKA ENGKLVINGK AITIFQERDP ANIKWGDAGA EYVVESTGVF TTMEKAGAHL KGGAKRVIIS APSADAPMFV MGVNHEKYNN TLKIVSNASC TTNCLAPLAK VIHDHFGIVE GLMTTVHAIT ATQKTVDGPS GKLWRDGRGA AQNIIPASTG AAKAVGKVIP ELNGKLTGMA FRVPTPNVSV VDLTCRLEKP AKYDEIKKVV KQASEGPLKG ILGYTEDQVV SCDFNSDTHS STFDAGAGIA LNDHFVKLIS WYDNEFGYSN RVVDLMVHMA SKE
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.
  • 基因功能參考文獻：
    1. GAPDH with CytOx could be the point of interest as it has already been shown that GAPDH protein damage results in a marked decrease in cellular ATP levels PMID: 24059522
    2. present in, and accounts for, glycolysis and glutamate accumulation into synaptic vesicles PMID: 12488440
    3. GAPDH phosphorylates the long intracellular loop of GABA(A) receptor alpha1 subunit at Ser-416 & Thr-337. The neuronal membrane-attached GAPDH is a dual-purpose enzyme, a glycolytic dehydrogenase, & a receptor-associated kinase. PMID: 15342727
    4. Results show that expression of GAPD increased during the late fattening stage of of hanwoo steers and it is involved in metabolic pathways such as glycolysis and the TCA cycle. PMID: 17927910
  • 亞細胞定位：
    Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Nucleus.
  • 蛋白家族：
    Glyceraldehyde-3-phosphate dehydrogenase family
  • 數據庫鏈接：


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