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  4. Recombinant Bovine Diacylglycerol O-acyltransferase 1 (DGAT1), partial

Recombinant Bovine Diacylglycerol O-acyltransferase 1 (DGAT1), partial

  • 中文名稱：
    牛DGAT1重組蛋白
  • 貨號：
    CSB-YP836615BO1
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    牛DGAT1重組蛋白
  • 貨號：
    CSB-EP836615BO1
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    牛DGAT1重組蛋白
  • 貨號：
    CSB-EP836615BO1-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    牛DGAT1重組蛋白
  • 貨號：
    CSB-BP836615BO1
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    牛DGAT1重組蛋白
  • 貨號：
    CSB-MP836615BO1
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    DGAT1
  • Uniprot No.：
  • 別名：
    DGAT1; Diacylglycerol O-acyltransferase 1; Acyl-CoA retinol O-fatty-acyltransferase; ARAT; Retinol O-fatty-acyltransferase; Diglyceride acyltransferase
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Highly expressed in epithelial cells of the small intestine and its activity is essential for the absorption of dietary fats. In liver, plays a role in esterifying exogenous fatty acids to glycerol, and is required to synthesize fat for storage. Also present in female mammary glands, where it produces fat in the milk. May be involved in VLDL (very low density lipoprotein) assembly. In contrast to DGAT2 it is not essential for survival. Functions as the major acyl-CoA retinol acyltransferase (ARAT) in the skin, where it acts to maintain retinoid homeostasis and prevent retinoid toxicity leading to skin and hair disorders. Exhibits additional acyltransferase activities, includin acyl CoA:monoacylglycerol acyltransferase (MGAT), wax monoester and wax diester synthases. Also able to use 1-monoalkylglycerol (1-MAkG) as an acyl acceptor for the synthesis of monoalkyl-monoacylglycerol (MAMAG).
  • 基因功能參考文獻：
    1. the effects of dietary linseed oil, DGAT1, and the interaction between dietary linseed oil and DGAT1 on CH4 and H2 emission, energy and N metabolism, lactation performance, ruminal fermentation, and rumen bacterial and archaeal composition of dairy cows PMID: 28918153
    2. Here, the association between DGAT1 K232A, SCD1 A293V, and LEPR T945M markers with milk fat composition in southern Chile was evaluated. The most frequent variants for DGAT1, SCD1, and LEPR polymorphisms were GC/GC, C, and C, respectively. The DGAT1 GC/GC allele was associated with lower milk fat and protein content, lower saturated fatty acid levels, and higher polyunsaturated FA PMID: 27173340
    3. study showed that the DGAT1 K232A polymorphism has major effects on FA and mineral composition of bovine milk; effects are relevant in relation to cow health, human health, and functional properties of milk fat and milk protein PMID: 26898284
    4. Association of a novel mutation in DGAT1 that may be responsible for unsaturated milk fat in cattle. PMID: 25719731
    5. An effect of DGAT1 identified on subcutaneous fat thickness. PMID: 25414103
    6. Russian and Kazakh populations of the Kazakh white-headed breed were characterized by a high content of the AA genotype of RORC (0.713 and 0.608, respectively) and of the AA genotype of DGAT1 (0.913 and 0.975) PMID: 25975152
    7. Studied the occurrence of the DGAT1 p.Lys232Ala polymorphism in Romanian Holstein cattle and Romanian Buffalo breeds and further investigated its possible influence on fat percentage and fatty acid profiles. PMID: 25380462
    8. Novel SNPs and INDEL polymorphism in DGAT1 are associated with milk production and protein and fat milk composition. PMID: 24676595
    9. Single nucleotide polymorphisms in the DGAT1 gene significantly effected fat distribution in Chinese commercial cattle. PMID: 23143182
    10. effects of K232A polymorphism on milk fat composition in winter and summer PMID: 23127906
    11. results indicated that knockdown of DGAT1 expression significantly reduced triglyceride content in bovine mammary epithelial cells PMID: 23539897
    12. The use of leptin, leptin receptor, and DGAT1 polymorphisms as markers within genetic selection programs to improve and adjust several compositional parameters. PMID: 22127264
    13. pleiotropic effects of polymorphism Ala to Lys amino acid substitution at position 232 in mammary gland tissue PMID: 22916903
    14. The impact of different alleles of DGAT1 and leptin on milk production in Giorlando cattle is reported. PMID: 21396671
    15. Genotype KK has the greatest effect on all milk composition content traits, while genotype AA has the greatest effect on yield traits. It can be concluded that the DGAT1 gene can be used as a gene marker for assisted selection in milk composition traits. PMID: 21881942
    16. No differential allelic expression was observed for the DGAT1 genes in bovine fetal tissues. PMID: 21899958
    17. The present study investigated putative interaction effects between the DGAT1 K232A mutation and the polygenic term (i.e., all genes except DGAT1) for 5 milk production traits in the German Holstein dairy cattle population. PMID: 21338826
    18. The K232A substitution of the DGAT1 gene could be used as a golden standard in comparisons of the effect on milk productivity for the total gene set. PMID: 21446190
    19. The results confirm the lack of an association found by many other studies and suggest that these SNPs are not influential on the divergent intramuscular fat levels in the crossbred population tested. PMID: 20416823
    20. Effects of the DGAT1 p.Lys232Ala on milk, fat and protein yield, as well as fat and protein percentage in the milk of 1222 Holstein cows showed DGAT1 allele that encode lysine at position 232 was associated with increased 305-day milk fat yield. PMID: 19781040
    21. The effects of an amino acid substitution in DGAT1 on milk yield in Holstein cattle, and its frequency in North American cattle, are reported. PMID: 20379885
    22. DGAT1 polymorphism had the strongest association with milk FA composition, which confirmed the key role of DGAT1 in lipid metabolism of mammary gland PMID: 20105547
    23. results suggested a potential effect of the DGAT1 polymorphism on nonreturn rates for insemination 28 and 56 d after the first service PMID: 19841232
    24. K allele, causing an increase in milk fat percentage in the live animal, is characterized by a higher Vmax in producing triglycerides than the A allele. PMID: 14983021
    25. examined and characterized the occurrance of the K232A substitution in the DGAT1 gene in Bos taurus and Bos indicus PMID: 15190946
    26. Variation in the number of tandem repeats of the 18mer element might be causal for the variability in the transcription level of the DGAT1 gene. PMID: 15342525
    27. DGAT1 found to have greatest additive effect on subcutaneous fat thickness in beef cattle after Bayesian model selection analysis of 5 candidate genes PMID: 16175458
    28. associated with increased fat yield, fat and protein percent as well as decrease in milk yield; also in Bos indicus and Bubalus bubalis PMID: 17087837
    29. A simultaneous analysis of the influence of CYP11B1 and DGAT1 on multiple variables of a German Holstein pedigree is presented. PMID: 17179535
    30. characterization of both the VNTR and the K232A polymorphisms in the DGAT1 gene in the 3 main French dairy cattle breeds PMID: 17517739
    31. The SNP (C/T) detected at position 11785 in exon 17 creates a substitution change for the amino acid sequence, resulting in an Ala residue (GCG) transition to a Val residue (GTG) in position 484 of buffalo DGAT1 protein. PMID: 17592768
    32. we identified differences in the magnitude of effects between paternally and maternally inherited DGAT1 promoter VNTR - K232A haplotypes indicating parent-of-origin effects on milk production traits PMID: 17892573
    33. K232A polymorphism has a clear influence on milk-fat composition. PMID: 17894561
    34. bovine Acyl-CoA:diacylglycerol acyltransferase 1 (DGAT1) K232A polymorphism effects were found for fat and protein percentages and milk yield PMID: 18420644
    35. conclude that selective breeding can contribute to higher unsaturation indices, and that selective breeding can capitalize on genotypic information of both stearoyl-CoA desaturase (SCD1)and diacylglycerol O-acyltransferase homolog 1(DGAT1)polymorphism PMID: 18420645
    36. single nucleotide polymorphisms in Diacylglycerol acyltransferase had a significant effect on milk yield PMID: 18650297
    37. DGAT1 and growth hormone receptor alleles whichor increase in milk production were found to have an adverse effect on reproduction, while the leptin allele responsible for increase in milk production was linked to marginally increased metritis frequency. PMID: 18822099

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  • 亞細胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Membrane-bound acyltransferase family, Sterol o-acyltransferase subfamily
  • 數據庫鏈接：


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