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  4. Recombinant Bovine DNA polymerase delta subunit 4 (POLD4)

Recombinant Bovine DNA polymerase delta subunit 4 (POLD4)

  • 中文名稱：
    牛POLD4重組蛋白
  • 貨號：
    CSB-YP666210BO
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    牛POLD4重組蛋白
  • 貨號：
    CSB-EP666210BO
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    牛POLD4重組蛋白
  • 貨號：
    CSB-EP666210BO-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    牛POLD4重組蛋白
  • 貨號：
    CSB-BP666210BO
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    牛POLD4重組蛋白
  • 貨號：
    CSB-MP666210BO
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    POLD4; DNA polymerase delta subunit 4; DNA polymerase delta subunit p12
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-107
  • 氨基酸序列
    MGRKRLITDS YPVVKRREGS AGHSKGELAP DLGEEPLPLS VDEEELELLR QFDLAWQYGP CTGITRLQRW HRAEQMGLKP PPEVHQVLQS HPGDPRFQCS LWHFYPL
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    As a component of the tetrameric DNA polymerase delta 4 complex (Pol-delta4), plays a role in high fidelity genome replication and repair. Within this complex, increases the rate of DNA synthesis and decreases fidelity by regulating POLD1 polymerase and proofreading 3' to 5' exonuclease activity. Pol-delta4 participates in Okazaki fragment processing, through both the short flap pathway, as well as a nick translation system. Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR), a mechanism that may induce segmental genomic duplications of up to 200 kb. Involved in Pol-delta4 translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites. Its degradation in response to DNA damage is required for the inhibition of fork progression and cell survival.
  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    DNA polymerase delta subunit 4 family
  • 數據庫鏈接：


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