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  4. Recombinant Bovine Arrestin-C (ARR3)

Recombinant Bovine Arrestin-C (ARR3)

  • 中文名稱：
    Recombinant Bovine Arrestin-C(ARR3)
  • 貨號：
    CSB-YP878783BO
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Bovine Arrestin-C(ARR3)
  • 貨號：
    CSB-EP878783BO
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Bovine Arrestin-C(ARR3)
  • 貨號：
    CSB-EP878783BO-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Bovine Arrestin-C(ARR3)
  • 貨號：
    CSB-BP878783BO
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Bovine Arrestin-C(ARR3)
  • 貨號：
    CSB-MP878783BO
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ARR3
  • Uniprot No.：
  • 別名：
    ARR3Arrestin-C; Cone arrestin; cArr; Retinal cone arrestin-3; X-arrestin
  • 種屬：
    Bos taurus (Bovine)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-389
  • 氨基酸序列
    MSRVFKKTCS NGKLSIYLGK RDFVDHVDMV EPIDGVVLVD PEYLKGRKMF VMLTCAFRYG HDDLDVIGLT FRKDLYVQVQ QVVPAESSSP RGSLTVLQER LLHKLGDNAY PFTLQMVVNL PCSVTLQPGP DDTGKACGVD FEVKSFCAEN LEEKVSKRDS VRLVIRKIQF APLEPGPGPW ARLCRRFLLS AQPLLLQAWM DKEVNYHGQP ISVNVSINNS TNKVIKKIKI SVDQITDVVL YSLDKYTKTV FVQEFTETIA ANSTFSKSFA VTPLLADNCH KQGLALDGKL KQGDTNLASS TILRPGVDKE LLGILVSYKV RVNLMVSCEG ILGDLTASEV GVELPLILMH PKPSNEAASS EDIVIEEFAQ QEPSGESQEA LAAEGNEGS
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    May play a role in an as yet undefined retina-specific signal transduction. Could bind to photoactivated-phosphorylated red/green opsins.
  • 基因功能參考文獻：
    1. Inositol hexakisphosphate (IP6) binds to the arrestin-3 phosphate sensor, and is stabilized by trimerization. Analysis of the trimerization surface, which is also the receptor-binding surface, suggests a feature called the finger loop as a key region of the activation sensor. PMID: 29127291
    2. Several mutations in the two parts of the central "crest" of the arrestin molecule, middle-loop and C-loop, enhanced or reduced arrestin-3 interactions with several GPCRs in receptor subtype and functional state-specific manner. PMID: 28461104
    3. K2A mutations in arrestin-1, -2, and -3 significantly reduced their binding to active phosphorhodopsin. PMID: 22275358
    4. multiple residues on the non-receptor-binding side of arrestin-3 are crucial for JNK3 activation PMID: 21715332
    5. Both nonvisual arrestin-2 and arrestin-3 are shown to directly bind Jun kinase (JNK)3alpha2 and its upstream activator Map kinase kinase (MKK)4; the affinity of arrestin-3 for these kinases is higher than that of arrestin-2. PMID: 22047447
    6. the first crystal structure of arrestin-3, solved at 3.0 A resolution. Receptor binding. PMID: 21215759
    7. SUMOylation sites in arrestin-3; arrestin-3 SUMOylation mediates beta(2)AR internalization PMID: 21118812
    8. The nature of the changes in arrestin 3 distribution observed upon activation of adenosine receptors correlates with receptor sensitivity to G-protein-coupled receptor kinase-mediated phosphorylation and rapid internalization. PMID: 12475223
    9. microtubule interaction may play a role in keeping p44 arrestin away from rhodopsin in dark-adapted photoreceptors PMID: 15272005

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  • 亞細胞定位：
    Photoreceptor inner segment. Cell projection, cilium, photoreceptor outer segment.
  • 蛋白家族：
    Arrestin family
  • 組織特異性：
    Expressed in cone photoreceptors in the retina (at protein level).
  • 數據庫鏈接：

    KEGG: bta:281011

    STRING: 9913.ENSBTAP00000017088

    UniGene: Bt.494



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