Recombinant Bovine Alpha-crystallin B chain (CRYAB)

^{In Stock}

貨號：

CSB-EP006008BO
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

CRYAB
Uniprot No.：

P02510
種屬：

Bos taurus (Bovine)
蛋白長度：

Full Length
來源：

E.coli
分子量：

27.5 kDa
表達區域：

1-175aa
氨基酸序列

MDIAIHHPWIRRPFFPFHSPSRLFDQFFGEHLLESDLFPASTSLSPFYLRPPSFLRAPSWIDTGLSEMRLEKDRFSVNLDVKHFSPEELKVKVLGDVIEVHGKHEERQDEHGFISREFHRKYRIPADVDPLAITSSLSSDGVLTVNGPRKQASGPERTIPITREEKPAVTAAPKK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions.
基因功能參考文獻：
1. analysis of the structural, molecular and spectroscopic changes in alpha-crystallin protein as it slowly denatures due to aggregation induced by non-enzymatic glycation PMID: 26215735
2. The alphaB-crystallin content of bovine type I muscle fibers is quantitatively similar to that in the predominantly type I fibers of rat soleus muscle. PMID: 21975426
3. These results show that between pH 7 and 10 the protein undergoes a reversible thermal transition. PMID: 21445944
4. alpha-crystallin is characterized by homogeneous distribution of scattering density in the domains inaccessible for water penetration PMID: 21314599
5. In the presence of alpha-crystallin or GroEL the kinetic of GAPDH aggregation is changed from the diffusion-limited cluster-cluster aggregation to the reaction-limited cluster-cluster aggregation. PMID: 19936900
6. one of the functions of alphaB-crystallin is to bind microtubules via microtubule binding proteins and to give the MTs resistance to disassembly PMID: 15075233
7. The study provides evidence for the regulation of the chaperone activity of alphaB-crystallin by phosphorylation and indicates that this may play an important role in alleviating the pathogenic effects associated with protein conformational diseases. PMID: 16928191
8. analysis of alpha-crystallin assisted refolding of enzyme substrates PMID: 17211683
9. The results are consistent with the hypothesis that short-range, weak, attractive interactions between alpha- and gamma-crystallins are necessary for maximum transparency of the lens. PMID: 18509547
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亞細胞定位：

Cytoplasm. Nucleus. Secreted.
蛋白家族：

Small heat shock protein (HSP20) family
組織特異性：

Lens as well as other tissues.
數據庫鏈接：

KEGG: bta:281719

STRING: 9913.ENSBTAP00000000556

UniGene: Bt.88059