在线日韩日本国产亚洲丨少妇伦子伦情品无吗丨欧美性猛交xxxx免费看蜜桃丨精品人妻系列无码一区二区三区丨亚洲精品无码不卡在线播放

Your Good Partner in Biology Research

Recombinant Bat coronavirus HKU5 Replicase polyprotein 1ab (rep), partial

  • 中文名稱:
    Recombinant Bat coronavirus HKU5 Replicase polyprotein 1ab(rep),partial
  • 貨號:
    CSB-YP313720BOU
  • 規格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    Recombinant Bat coronavirus HKU5 Replicase polyprotein 1ab(rep),partial
  • 貨號:
    CSB-EP313720BOU
  • 規格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    Recombinant Bat coronavirus HKU5 Replicase polyprotein 1ab(rep),partial
  • 貨號:
    CSB-EP313720BOU-B
  • 規格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    Recombinant Bat coronavirus HKU5 Replicase polyprotein 1ab(rep),partial
  • 貨號:
    CSB-BP313720BOU
  • 規格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    Recombinant Bat coronavirus HKU5 Replicase polyprotein 1ab(rep),partial
  • 貨號:
    CSB-MP313720BOU
  • 規格:
  • 來源:
    Mammalian cell
  • 其他:

產品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    rep
  • Uniprot No.:
  • 別名:
    rep; 1a-1b; Replicase polyprotein 1ab; pp1ab; ORF1ab polyprotein) [Cleaved into: Host translation inhibitor nsp1; nsp1; Leader protein); Non-structural protein 2; nsp2; p65 homolog); Papain-like proteinase; PL-PRO; EC 3.4.19.12; EC 3.4.22.69; Non-structural protein 3; nsp3); Non-structural protein 4; nsp4); 3C-like proteinase; 3CL-PRO; 3CLp; EC 3.4.22.-; nsp5); Non-structural protein 6; nsp6); Non-structural protein 7; nsp7); Non-structural protein 8; nsp8); Non-structural protein 9; nsp9); Non-structural protein 10; nsp10; Growth factor-like peptide; GFL); RNA-directed RNA polymerase; Pol; RdRp; EC 2.7.7.48; nsp12); Helicase; Hel; EC 3.6.4.12; EC 3.6.4.13; nsp13); Guanine-N7 methyltransferase; ExoN; EC 2.1.1.-; EC 3.1.13.-; nsp14); Uridylate-specific endoribonuclease; EC 3.1.-.-; NendoU; nsp15); 2'-O-methyltransferase; EC 2.1.1.-; nsp16)]
  • 種屬:
    Bat coronavirus HKU5 (BtCoV) (BtCoV/HKU5/2004)
  • 蛋白長度:
    Partial
  • 蛋白標簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式:
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產品評價

靶點詳情

  • 功能:
    The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.; Inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.; May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.; Responsible for the cleavages located at the N-terminus of the replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Participates together with nsp4 in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF3. Prevents also host NF-kappa-B signaling.; Participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.; Cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes.; Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.; Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.; May participate in viral replication by acting as a ssRNA-binding protein.; Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.; Responsible for replication and transcription of the viral RNA genome.; Multi-functional protein with a zinc-binding domain in N-terminus displaying RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Activity of helicase is dependent on magnesium.; Enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity.; Plays a role in viral transcription/replication and prevents the simultaneous activation of host cell dsRNA sensors, such as MDA5/IFIH1, OAS, and PKR. Acts by degrading the 5'-polyuridines generated during replication of the poly(A) region of viral genomic and subgenomic RNAs. Catalyzes a two-step reaction in which a 2'3'-cyclic phosphate (2'3'-cP) is first generated by 2'-O transesterification, which is then hydrolyzed to a 3'-phosphate (3'-P). If not degraded, poly(U) RNA would hybridize with poly(A) RNA tails and activate host dsRNA sensors.; Methyltransferase that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system.
  • 亞細胞定位:
    [Papain-like proteinase]: Host membrane; Multi-pass membrane protein. Host cytoplasm.; [Non-structural protein 4]: Host membrane; Multi-pass membrane protein. Host cytoplasm.; [Non-structural protein 6]: Host membrane; Multi-pass membrane protein.; [Non-structural protein 7]: Host cytoplasm, host perinuclear region.; [Non-structural protein 8]: Host cytoplasm, host perinuclear region.; [Non-structural protein 9]: Host cytoplasm, host perinuclear region.; [Non-structural protein 10]: Host cytoplasm, host perinuclear region.; [Helicase]: Host endoplasmic reticulum-Golgi intermediate compartment.; [Uridylate-specific endoribonuclease]: Host cytoplasm, host perinuclear region.
  • 蛋白家族:
    Coronaviruses polyprotein 1ab family
  • 數據庫鏈接:

    KEGG: vg:4836003



主站蜘蛛池模板: 无码高潮喷水在线观看| 午夜福利精品亚洲不卡| 丰满的少妇xxxxx人| 欧洲亚洲色视频综合在线| 日本人妻巨大乳挤奶水| 麻豆精品国产精华精华液好用吗 | 好吊色欧美一区二区三区视频| 女人的精水喷出来视频| 精品国精品国产自在久国产不卡| 亚洲成av人片高潮喷水| 亚洲国产精品美女久久久久| 97超级碰碰人妻中文字幕 | 亚洲国产成人久久综合同性 | 成人无码www免费视频| 少妇挑战黑人高潮惨叫| 中文国产成人精品久久久 | 欧美牲交a欧美牲交aⅴ免费真 | 国产成人精品日本亚洲成熟| 少妇粉嫩小泬喷水视频www| 国产精品1区2区3区在线观看| 高潮毛片无遮挡高清免费视频| 嫩草欧美曰韩国产大片| 久久这里只有精品首页 | 2021最新精品国自产拍视频| 久草热久草热线频97精品| 成人免费视频一区二区三区| 免费观看的av在线播放| 国内精品久久久久久久影视麻豆| 少妇无码一区二区三区免费| 丁香色欲久久久久久综合网| 青青草国产线观看| 亚洲国产精品无码久久sm| 久久99国产亚洲高清观看首页| 性无码专区无码片| 国产一本一道久久香蕉| 国产伦孑沙发午休精品| 国产精品不卡无码av在线播放| aaa午夜级特黄日本大片| 无码人妻丰满熟妇区96| 欧美激情精品久久久久久| 日韩精品亚洲aⅴ在线影院|