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  4. Recombinant Bacillus subtilis Transcriptional regulator MntR (mntR)

Recombinant Bacillus subtilis Transcriptional regulator MntR (mntR)

  • 中文名稱：
    枯草芽孢桿菌mntR重組蛋白
  • 貨號：
    CSB-YP346178BRJ
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌mntR重組蛋白
  • 貨號：
    CSB-EP346178BRJ
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌mntR重組蛋白
  • 貨號：
    CSB-EP346178BRJ-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    枯草芽孢桿菌mntR重組蛋白
  • 貨號：
    CSB-BP346178BRJ
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌mntR重組蛋白
  • 貨號：
    CSB-MP346178BRJ
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    mntR
  • Uniprot No.：
  • 別名：
    mntR; yqhN; BSU24520; HTH-type transcriptional regulator MntR; Manganese transport regulator; Manganese(II) metalloregulatory protein MntR
  • 種屬：
    Bacillus subtilis (strain 168)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-142
  • 氨基酸序列
    MTTPSMEDYI EQIYMLIEEK GYARVSDIAE ALAVHPSSVT KMVQKLDKDE YLIYEKYRGL VLTSKGKKIG KRLVYRHELL EQFLRIIGVD EEKIYNDVEG IEHHLSWNSI DRIGDLVQYF EEDDARKKDL KSIQKKTEHH NQ
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Central regulator of manganese homeostasis that regulates the expression of both manganese uptake and efflux systems. In the presence of high levels of manganese, it mediates repression of the manganese uptake systems MntH and MntABCD and activation of the efflux systems MneP and MneS. Binds with high affinity to the regulatory regions of its target genes. The manganese concentration required for activation of efflux is higher than that for repression of uptake.
  • 基因功能參考文獻：
    1. Iron and manganese homeostasis in Bacillus subtilis involves three key regulators: Fur senses iron sufficiency, MntR senses manganese sufficiency, and PerR senses the intracellular Fe/Mn ratio. (Review) PMID: 25160631
    2. Experiments indicate that MntR undergoes a significant conformational change upon metal binding that results in stabilization of the protein structure. PMID: 15736948
    3. Electron Spin Resonance Spectroscopy spectra of the spin-labeled mutant indicate that tertiary structure is conserved in the presence or absence of bound metals, though slightly greater flexibility is present in inactive forms of MntR. PMID: 17118401
    4. MntR belongs to a class of metalloregulatory proteins that possess a weak affinity for its cognate metal ion, while still eliciting tight DNA binding upon complete metal loading. PMID: 17176058
  • 亞細胞定位：
    Cytoplasm.
  • 數據庫鏈接：


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