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  4. Recombinant Bacillus subtilis Replication initiation and membrane attachment protein (dnaB)

Recombinant Bacillus subtilis Replication initiation and membrane attachment protein (dnaB)

  • 中文名稱：
    枯草芽孢桿菌dnaB重組蛋白
  • 貨號：
    CSB-YP362263BRJ
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌dnaB重組蛋白
  • 貨號：
    CSB-EP362263BRJ
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌dnaB重組蛋白
  • 貨號：
    CSB-EP362263BRJ-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    枯草芽孢桿菌dnaB重組蛋白
  • 貨號：
    CSB-BP362263BRJ
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌dnaB重組蛋白
  • 貨號：
    CSB-MP362263BRJ
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    dnaB
  • Uniprot No.：
  • 別名：
    dnaB; BSU28990Replication initiation and membrane attachment protein
  • 種屬：
    Bacillus subtilis (strain 168)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-472
  • 氨基酸序列
    MADYWKDVLP VDPYVVKSRS MLQDIDRQII TQLYQPLIGP VAFSLYMTLW GELEQNRLWG GESTHRQLMG MTQSNLKTIH QEQGKLEGIG LLKVYMKESE RQERLFIYEL LPPLRPNEFF EDGMLNVFLY NRVGKTKYQQ LKQFFTHPAI SEDAKDITRP FNHAFESLQP SEWKLTSDME ETVRLAEGSE YTSVGQSPSY TITEDVFDFD LFLAGLSETM IPRKAMTQQV RDTIKKLSYL YGIDPLQMQN VVMSAIDERD VITTEALRKA ASDWYQIERN GQLPDLVEKT QPVHLREGEQ PAEEDSLDGK LIALLEAISP KKLLQDIADG TEPSKADLKI IEEIMFEQKL EPGVTNVLIY YVMLKTDMKL SKNYIQKIAS HWARKKVKTV REAMKLAIEE NRQYLEWAEG KTKSSKRNQK VIREEKLPDW MTEKETASDS ESGQQKLHPQ DLEEQKKKMM EEMQKLKKYS AY
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Probable component of primosome involved in the initiation of DNA replication. It is essential for both replication initiation and membrane attachment of the origin region of the chromosome and plasmid pUB110.
  • 基因功能參考文獻：
    1. Tetramerization of DnaB is mediated within residues 1-300, and DNA-dependent oligomerization within residues 365-428. PMID: 20071750
    2. intragenic suppressors should provide insights into structure-function relationships in DnaD and DnaB, and interactions between DnaD and DnaB PMID: 19707554
    3. results highlight the need for both DnaD and DnaB to interact individually and together with ssDNA during the early stages of initiation and re-initiation of chromosomal DNA replication PMID: 15686560
    4. Whereas DnaD opens up supercoiled DNA, DnaB acts as a lateral compaction protein. The two competing activities can act together on a supercoiled plasmid forming two topologically distinct poles; one compacted with DnaB and the other open with DnaD. PMID: 16002087
    5. propose a model whereby each DnaB monomer participates in both stacked components of the tetramer and displays a different overall shape PMID: 17289076

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