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Recombinant Bacillus subtilis Regulatory protein spx (spxA)

  • 中文名稱:
    枯草芽孢桿菌spxA重組蛋白
  • 貨號:
    CSB-YP521735BRJ
  • 說明書:
  • 規(guī)格:
  • 來源:
    Yeast
  • 其他:
  • 中文名稱:
    枯草芽孢桿菌spxA重組蛋白
  • 貨號:
    CSB-EP521735BRJ
  • 說明書:
  • 規(guī)格:
  • 來源:
    E.coli
  • 其他:
  • 中文名稱:
    枯草芽孢桿菌spxA重組蛋白
  • 貨號:
    CSB-EP521735BRJ-B
  • 說明書:
  • 規(guī)格:
  • 來源:
    E.coli
  • 共軛:
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他:
  • 中文名稱:
    枯草芽孢桿菌spxA重組蛋白
  • 貨號:
    CSB-BP521735BRJ
  • 說明書:
  • 規(guī)格:
  • 來源:
    Baculovirus
  • 其他:
  • 中文名稱:
    枯草芽孢桿菌spxA重組蛋白
  • 貨號:
    CSB-MP521735BRJ
  • 說明書:
  • 規(guī)格:
  • 來源:
    Mammalian cell
  • 其他:

產(chǎn)品詳情

  • 純度:
    >85% (SDS-PAGE)
  • 基因名:
    spxA
  • Uniprot No.:
  • 別名:
    spxA; yjbD; BSU11500; Regulatory protein Spx
  • 種屬:
    Bacillus subtilis (strain 168)
  • 蛋白長度:
    full length protein
  • 表達(dá)區(qū)域:
    1-131
  • 氨基酸序列
    MVTLYTSPSC TSCRKARAWL EEHEIPFVER NIFSEPLSID EIKQILRMTE DGTDEIISTR SKVFQKLNVN VESMPLQDLY RLINEHPGLL RRPIIIDEKR LQVGYNEDEI RRFLPRKVRS FQLREAQRLA N
  • 蛋白標(biāo)簽:
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式:
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng):
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet :
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能:
    Global transcriptional regulator that plays a key role in stress response and exerts either positive or negative regulation of genes. Acts by interacting with the C-terminal domain of the alpha subunit of the RNA polymerase (RNAP). This interaction can enhance binding of RNAP to the promoter region of target genes and stimulate their transcription, or block interaction of RNAP with activator proteins and repress transcription. Exhibits no DNA-binding activity.; Induces the expression of a large number of genes in response to a variety of stress conditions, such as disulfide, heat and cell wall stress, while concurrently repressing transcription of genes involved in various developmental and growth-related pathways during periods of extreme stress. Functions in the oxidative stress response via induction of the transcription of thioredoxin (trxA) and thioredoxin reductase (trxB) during thiol-specific oxidative (disulfide) stress. Mediates response to oxidative stress caused by paraquat (PQ) via induction of the methionine sulfoxide reductase genes, msrA and msrB. Also acts as a transcriptional activator of the bacillithiol (BSH) biosynthesis genes in response to oxidizing conditions and thio-reactive compounds. Involved in heat stress response and thermotolerance development, which results in diminished cellular protein aggregates. Plays an important adaptive role in the cell wall stress response. Participates in sulfate-dependent control of organosulfur metabolism. Negatively controls, via CymR, the expression of the organosulfur utilization operons ytmI, yxeI and ssu, and directly activates yrrT operon expression during growth in medium containing methionine as sole sulfur source. Negatively affects competence and sporulation. Inhibits biofilm formation in response to disulfide stress by repressing biofilm matrix genes.
  • 基因功能參考文獻(xiàn):
    1. Identification of 283 discrete chromosomal sites potentially bound by the Spx-RNA polymerase (Spx-RNAP) complex using chromatin immunoprecipitation of Spx. PMID: 22904090
    2. GtYjbH enhances ClpXP-catalysed proteolysis of Spx. The C terminus of Spx is required for YjbH binding. PMID: 22343351
    3. Oxidative stress induced the expression of msrAB partially through an oxidation on Spx via modification of its CXXC motif. PMID: 18662407
  • 亞細(xì)胞定位:
    Cytoplasm.
  • 蛋白家族:
    ArsC family, Spx subfamily
  • 數(shù)據(jù)庫鏈接:


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