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  4. Recombinant Bacillus subtilis Regulatory protein ribR (ribR)

Recombinant Bacillus subtilis Regulatory protein ribR (ribR)

  • 中文名稱：
    枯草芽孢桿菌ribR重組蛋白
  • 貨號：
    CSB-YP311327BRJ
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌ribR重組蛋白
  • 貨號：
    CSB-EP311327BRJ
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌ribR重組蛋白
  • 貨號：
    CSB-EP311327BRJ-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    枯草芽孢桿菌ribR重組蛋白
  • 貨號：
    CSB-BP311327BRJ
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌ribR重組蛋白
  • 貨號：
    CSB-MP311327BRJ
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ribR
  • Uniprot No.：
  • 別名：
    ribR; ytnK; BSU29300; RNA-binding riboflavin kinase RibR; EC 2.7.1.26
  • 種屬：
    Bacillus subtilis (strain 168)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-230
  • 氨基酸序列
    MTIIAGTVVK GKQLGRKLGF PTANVDAKIH GLRNGVYGVL ATVNHQFHLG VMNIGVKPTV GSNLEKTLEI FLFDFHRDIY GEKIECSILF KIREERRFDS LESLTKQIKK DISCVAKRFE LIGIMAPNKK ESLLSHQELN LPDLCFYKKC NNLYGVNRGV YNVIDNWFFE YGITQVAYRR IYILSFLSFL KEDNPKVSSK YIRFGAGGLA DKLNRFISSY VEESEENILG
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    May be directly involved in the regulation of the rib genes. C-terminal part of RibR specifically binds to RFN of the rib leader of the riboflavin biosynthetic operon. The RFN element is a sequence within the rib-leader mRNA reported to serve as a receptor for an FMN-dependent riboswitch. Possibly, RibR produces the comodulator FMN through its own N-terminal flavokinase activity. FMN-activated RibR may stabilize the anti-anti terminator structure of RFN mRNA, causing transcription termination of the rib genes in trans.
  • 基因功能參考文獻：
    1. RibR counteracts the FMN-induced "turn-off" activities of both FMN riboswitches in Bacillus subtilis, allowing rib gene expression even in the presence of high levels of FMN. PMID: 26494285
    2. Two sites of single nucleotide substitutions leading to derepresson of ribR gene were identified between the putative main promoter and transcription start of the ytlI-ytnM operon regulatory region. PMID: 15272571
    3. RibR specifically interacts in vivo with the nontranslated wild-type leader of the mRNA of the riboflavin biosynthetic operon. PMID: 17590224
  • 蛋白家族：
    RibR family
  • 數據庫鏈接：


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