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  4. Recombinant Bacillus subtilis RNA polymerase sigma factor rpoD (sigA)

Recombinant Bacillus subtilis RNA polymerase sigma factor rpoD (sigA)

  • 中文名稱：
    枯草芽孢桿菌sigA重組蛋白
  • 貨號(hào)：
    CSB-YP361751BRJ
  • 規(guī)格：
  • 來(lái)源：
    Yeast
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌sigA重組蛋白
  • 貨號(hào)：
    CSB-EP361751BRJ
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌sigA重組蛋白
  • 貨號(hào)：
    CSB-EP361751BRJ-B
  • 規(guī)格：
  • 來(lái)源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    枯草芽孢桿菌sigA重組蛋白
  • 貨號(hào)：
    CSB-BP361751BRJ
  • 規(guī)格：
  • 來(lái)源：
    Baculovirus
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌sigA重組蛋白
  • 貨號(hào)：
    CSB-MP361751BRJ
  • 規(guī)格：
  • 來(lái)源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    sigA
  • Uniprot No.：
  • 別名：
    sigA; rpoD; BSU25200; RNA polymerase sigma factor SigA; Sigma-43; Sigma-A
  • 種屬：
    Bacillus subtilis (strain 168)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-371
  • 氨基酸序列
    MADKQTHETE LTFDQVKEQL TESGKKRGVL TYEEIAERMS SFEIESDQMD EYYEFLGEQG VELISENEET EDPNIQQLAK AEEEFDLNDL SVPPGVKIND PVRMYLKEIG RVNLLSAKEE IAYAQKIEEG DEESKRRLAE ANLRLVVSIA KRYVGRGMLF LDLIQEGNMG LMKAVEKFDY RKGYKFSTYA TWWIRQAITR AIADQARTIR IPVHMVETIN KLIRVQRQLL QDLGREPTPE EIAEDMDLTP EKVREILKIA QEPVSLETPI GEEDDSHLGD FIEDQEATSP SDHAAYELLK EQLEDVLDTL TDREENVLRL RFGLDDGRTR TLEEVGKVFG VTRERIRQIE AKALRKLRHP SRSKRLKDFL E
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Sigma factors are initiation factors that promote the attachment of RNA polymerase (RNAP) to specific initiation sites and are then released. This sigma factor is the primary sigma factor during all growth phases, stress and during sporulation. It associates with the RNAP core at all growth stages with a slight increase in stationary phase.
  • 基因功能參考文獻(xiàn)：
    1. Data suggest that domain 1.1 of SigA requires minimal conformational changes for binding to RNAP core in the DNA channel. (SigA = sigma factor from Bacillus subtilis; RNAP core = RNA polymerase from Thermotoga maritima, core is composed of 5 subunits: 2 alpha, beta, beta-prime, omega) PMID: 28539362
    2. Analysis of the sequence in proximity of the transcription start site revealed a sequence suitable for the housekeeping sigma(A) subunit of RNA polymerase. PMID: 16964327
  • 亞細(xì)胞定位：
    Cytoplasm.
  • 蛋白家族：
    Sigma-70 factor family, RpoD/SigA subfamily
  • 數(shù)據(jù)庫(kù)鏈接：


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