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  4. Recombinant Bacillus subtilis Peptidoglycan DL-endopeptidase CwlO (cwlO)

Recombinant Bacillus subtilis Peptidoglycan DL-endopeptidase CwlO (cwlO)

  • 中文名稱：
    Recombinant Bacillus subtilis Peptidoglycan DL-endopeptidase CwlO(cwlO)
  • 貨號：
    CSB-YP331220BRJ
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Bacillus subtilis Peptidoglycan DL-endopeptidase CwlO(cwlO)
  • 貨號：
    CSB-EP331220BRJ
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Bacillus subtilis Peptidoglycan DL-endopeptidase CwlO(cwlO)
  • 貨號：
    CSB-EP331220BRJ-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Bacillus subtilis Peptidoglycan DL-endopeptidase CwlO(cwlO)
  • 貨號：
    CSB-BP331220BRJ
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Bacillus subtilis Peptidoglycan DL-endopeptidase CwlO(cwlO)
  • 貨號：
    CSB-MP331220BRJ
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    cwlO
  • Uniprot No.：
  • 別名：
    cwlO; yvcE; yzkA; BSU34800Peptidoglycan DL-endopeptidase CwlO; EC 3.4.-.-; D-gamma-glutamyl-meso-diaminopimelyl DL-endopeptidase; PSPA2
  • 種屬：
    Bacillus subtilis (strain 168)
  • 蛋白長度：
    full length protein
  • 表達區域：
    31-473
  • 氨基酸序列
    ETLDEKKQKI ESKQSEVASS IEAKEKELTE LQENQSKIEK ELKDINDKAL DTSNKIEDKK EENDKTKEEI KKLKKEIKET EARIEKRNEI LKKRVRSLQE SGGSQGYIDV LLGSTSFGDF ISRATAVSSI VDADKDLIKQ QEQDKAKLED SEADLNDKLK EVQAALAKLE TMQKDLDKQL NEKDKLFDEA KASQKKTAKA ISELKSEASE LANQKANTEA EQARIKKEQE AAAALIKKQE EAQKASDETQ TDDSQTATTE SSKASSSDDS SDNSSDNSSN GSSNSSSNGS SSKKSSGSNS NSGGTVISNS GGIEGAISVG SSIVGQSPYK FGGGRTQSDI NNRIFDCSSF VRWAYASAGV NLGPVGGTTT DTLVGRGQAV SASEMKRGDL VFFDTYKTNG HVGIYLGNGT FLNDNTSHGV SVDSMSNPYW KAAFKGVVRR VVQ
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    The C-terminal part of CwlO shows a cell wall hydrolytic DL-endopeptidase activity.
  • 基因功能參考文獻：
    1. Here authors show that activity of CwlO is regulated by an ABC transporter, FtsEX, which is required for cell elongation, unlike cell division as in Escherichia coli. PMID: 23869552
    2. The cwlO transcript is very unstable, with its degradation initiated by RNase Y cleavage within the 187-nucleotide leader sequence. PMID: 24163346
    3. Endopeptidase activity of LytE or CwlO is essential for cell proliferation. These results strongly suggest that LytE and CwlO are involved in cell elongation and support the inside-to-outside model for peptidoglycan sacculus formation. PMID: 22139507
  • 亞細胞定位：
    Secreted. Secreted, cell wall.
  • 蛋白家族：
    Peptidase C40 family
  • 數據庫鏈接：


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