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Recombinant Bacillus subtilis Penicillin-binding protein 4 (pbpD), partial

In Stock
  • 中文名稱(chēng):
    枯草芽孢桿菌pbpD重組蛋白
  • 貨號(hào):
    CSB-EP334764BRJ
  • 規(guī)格:
    ¥2328
  • 圖片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP334764BRJ could indicate that this peptide derived from E.coli-expressed Bacillus subtilis (strain 168) pbpD.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP334764BRJ could indicate that this peptide derived from E.coli-expressed Bacillus subtilis (strain 168) pbpD.
  • 其他:

產(chǎn)品詳情

  • 純度:
    Greater than 90% as determined by SDS-PAGE.
  • 基因名:
    pbpD
  • Uniprot No.:
  • 別名:
    pbpD; BSU31490; Penicillin-binding protein 4; PBP 4) [Includes: Penicillin-insensitive transglycosylase; EC 2.4.1.129; Peptidoglycan TGase); Penicillin-sensitive transpeptidase; EC 3.4.16.4; DD-transpeptidase)]
  • 種屬:
    Bacillus subtilis (strain 168)
  • 蛋白長(zhǎng)度:
    Partial
  • 來(lái)源:
    E.coli
  • 分子量:
    43.0kDa
  • 表達(dá)區(qū)域:
    213-450aa
  • 氨基酸序列
    PNNPTLYDPLKHFDYTKSRQERLLKGLKDAGVITDKELKKAVKQKIKLDVEKREDKYPDYVSYVNDEFTQLVSESEGFDKRLQKASGKQKEKIENELSARVSTLMKDGVKIYTALDPYMQNQVVAQMNSKLPYADVQGGAAVINHQTHQIIALSGGKNYQKYDFNRAYQAYRQPGSSIKPLLDYGPYIEQTGATTSSTIDASKFCSKDYCPQNYNNRTYGTVTLDTAFKNSYNTPAIR
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽:
    N-terminal 6xHis-SUMO-tagged
  • 產(chǎn)品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
    Note: If you have any special requirement for the glycerol content, please remark when you place the order.
    If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 儲(chǔ)存條件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期:
    3-7 business days
  • 注意事項(xiàng):
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 產(chǎn)品描述:

    Recombinant B. subtilis pbpD expression in E.coli requires the insertion of the target DNA fragment into an E.coli expression vector, routinely a plasmid vector, and the transferral of this vector into E.coli cells. The cells are then cultured and induced to express this pbpD protein.?The cells are harvested by centrifugation, samples prepared and proteins detected by polyacrylamide gel electrophoresis and subsequent staining of the gel with Coomassie Brilliant Blue or silver stain or by immunoblotting. The pbpD protein expression in E.coli?is highly scalable and can be adjusted from the bacterial colony to conical flasks for liquid cultures, to fermentation reaction chambers.

    Penicillin-binding protein 4 (pbpD, PBP4) can promote antibiotic resistance independently of PBP2a. Altered expression of PBP4 is at least partially responsible for the VISA phenotype. PBP4 activity and regulation appear to be important for the response of S. aureus to vancomycin. PBP4 is associated with low levels of resistance to β-lactam antibiotics, but the mechanism for this is unclear. PBP4 plays a role in cell wall biosynthesis along with multiple PBPs. Overexpression of PBP4 is associated with low levels of methicillin resistance.

  • Datasheet & COA:
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能:
    Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) (Probable). Has a partially redundant function with PBP-2A (pbpA) during spore outgrowth.
  • 亞細(xì)胞定位:
    Cell membrane; Peripheral membrane protein.
  • 蛋白家族:
    Glycosyltransferase 51 family; Transpeptidase family
  • 數(shù)據(jù)庫(kù)鏈接:


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