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  4. Recombinant Bacillus subtilis Octanoyl-[GcvH]:protein N-octanoyltransferase (lipL)

Recombinant Bacillus subtilis Octanoyl-[GcvH]:protein N-octanoyltransferase (lipL)

  • 中文名稱：
    枯草芽孢桿菌lipL重組蛋白
  • 貨號：
    CSB-YP331056BRJ
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌lipL重組蛋白
  • 貨號：
    CSB-EP331056BRJ
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌lipL重組蛋白
  • 貨號：
    CSB-EP331056BRJ-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    枯草芽孢桿菌lipL重組蛋白
  • 貨號：
    CSB-BP331056BRJ
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌lipL重組蛋白
  • 貨號：
    CSB-MP331056BRJ
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    lipL
  • Uniprot No.：
  • 別名：
    lipL; ywfL; BSU37640; ipa-90d; Octanoyl-[GcvH]:protein N-octanoyltransferase; EC 2.3.1.204; Octanoyl-[GcvH]:E2 amidotransferase
  • 種屬：
    Bacillus subtilis (strain 168)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-281
  • 氨基酸序列
    MANQPIDLLM QPKWRVIDQS SLGPLFDAKQ SFAMDDTLCM SVGKGVSPAT ARSWVHHDTI VLGIQDTRLP FLQDGISLLE SEGYRVIVRN SGGLAVVLDD GVLNISLIFE DEKKGIDIDK GYEAMVELMR RMLRPYNAKI EAYEIEGSYC PGSYDLSING KKFAGISQRR VRGGVAVQIY LCADKSGSER ADLIRRFYQA ALKDKQNDKK GVYPEIRPET MASLSELLQK DISVQDLMFA LLTELKALST HLYSAGLSID EEMEFEKNLV RMAERNAKVF G
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Catalyzes the amidotransfer (transamidation) of the octanoyl moiety from octanoyl-GcvH to the lipoyl domain of the E2 subunit of lipoate-dependent enzymes.
  • 基因功能參考文獻：
    1. B. subtilis delta-lipL (ywfL) strains are unable to synthesize lipoic acid. They require additional supplements to grow on glucose minimal media and exhibit a growth defect on rich media. PMID: 21338420
    2. The product of this gene catalyses the amidotransfer (transamidation) of the octanoyl moiety from octanoyl-GcvH to the E2 subunit of pyruvate dehydrogenase. LipL activity was demonstrated in vitro with purified components and proceeds via a thioester-link PMID: 21338421
  • 蛋白家族：
    Octanoyltransferase LipL family
  • 數據庫鏈接：


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