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  4. Recombinant Bacillus subtilis Killing factor sdpC (sdpC)

Recombinant Bacillus subtilis Killing factor sdpC (sdpC)

  • 中文名稱：
    枯草芽孢桿菌sdpC重組蛋白
  • 貨號：
    CSB-YP520198BRJ
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌sdpC重組蛋白
  • 貨號：
    CSB-EP520198BRJ
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌sdpC重組蛋白
  • 貨號：
    CSB-EP520198BRJ-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    枯草芽孢桿菌sdpC重組蛋白
  • 貨號：
    CSB-BP520198BRJ
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌sdpC重組蛋白
  • 貨號：
    CSB-MP520198BRJ
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    sdpC
  • Uniprot No.：
  • 別名：
    sdpC; yvaY; BSU33770; Sporulation delaying protein C; SdpC; Cannibalism toxin SDP; Killing factor SdpC; Toxic peptide SdpC) [Cleaved into: Sporulation delaying protein; SDP)]
  • 種屬：
    Bacillus subtilis (strain 168)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    33-203
  • 氨基酸序列
    KENHTFSG EDYFRGLLFG QGEVGKLISN DLDPKLVKEA NSTEGKKLVN DVVKFIKKDQ PQYMDELKQS IDSKDPKKLI ENMTKADQLI QKYAKKNENV KYSSNKVTPS CGLYAVCVAA GYLYVVGVNA VALQTAAAVT TAVWKYVAKY SSSASNNSDL EAAAAKTLKL IHQ
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Produces a 42-residue extracellular sporulation delaying protein (SDP) that collapses the proton motive force (probably both the membrane potential and pH gradient) across the cell membrane, which leads to autolysis; may form a proton channel. Induces the lysis of other B.subtilis cells that have not entered the sporulation pathway, inducing cannibalism to provide a source of nutrients to support sporulation, and at the same time delaying commitment to the energetically expensive and irreversible onset of sporulation. Addition of SDP to liquid cultures halts growth, leads to increased cell permeability and eventually cell lysis in a significant subset of the population, although some cells survive and resume growth after a lag period. Effects of SDP are irreversible within 10 minutes. Addition of SDP to solid cultures induces killing, it is much more effective than SKF (AC O31422). Has antibiotic action against Gram-positive Firmicutes (L.acidophilus, M.megaterium, P.polymyxa, S.aureus, S.epidermidis) but not Actinobacteria M.luteus or Gram-negative P.aeruginosa or K.pneumoniae. SDP induces expression of the sdpR-sdpI operon. Its maturation is dependent on SdpA and SdpB. Also functions as a ligand, binds to SdpI triggering a signal transduction cascade that protects the cell against the toxic effects of its own SDP.
  • 基因功能參考文獻：
    1. These data suggest that SDP toxin production is a multistep process and that SdpAB are required for SDP production likely by controlling, directly or indirectly, cleavage of SDP from the pro-SdpC precursor. PMID: 23687264
  • 亞細胞定位：
    Secreted. Note=Produces a secreted protein originating from this gene (PubMed:12817086), secreted by the general secretory pathway (PubMed:14568161).
  • 數據庫鏈接：


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