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  4. Recombinant Bacillus subtilis HTH-type transcriptional regulator tnrA (tnrA)

Recombinant Bacillus subtilis HTH-type transcriptional regulator tnrA (tnrA)

  • 中文名稱：
    枯草芽孢桿菌tnrA重組蛋白
  • 貨號：
    CSB-YP670457BRJ
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌tnrA重組蛋白
  • 貨號：
    CSB-EP670457BRJ
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌tnrA重組蛋白
  • 貨號：
    CSB-EP670457BRJ-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    枯草芽孢桿菌tnrA重組蛋白
  • 貨號：
    CSB-BP670457BRJ
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    枯草芽孢桿菌tnrA重組蛋白
  • 貨號：
    CSB-MP670457BRJ
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    tnrA
  • Uniprot No.：
  • 別名：
    tnrA; scgR; BSU13310; HTH-type transcriptional regulator TnrA; Transcriptional nitrogen regulatory protein A; TnrA
  • 種屬：
    Bacillus subtilis (strain 168)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-110
  • 氨基酸序列
    MTTEDHSYKD KKVISIGIVS ELTGLSVRQI RYYEERKLIY PQRSSRGTRK YSFADVERLM DIANKREDGV QTAEILKDMR KKEQMLKNDP QVRKKMLEGQ LNAHFRYKNR
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Transcription regulator that actives the transcription of genes required for nitrogen assimilation such as nrgAB (ammonium transport), nasABCDEF (nitrate/nitrite assimilation), ureABC (urea degradation) and gabP (GABA transport), during nitrogen limitation. Also represses glnRA and gltAB in the absence of ammonium. On the contrary of the MerR members, which require longer DNA sites for high-affinity binding, TnrA requires a DNA sequence of 17 nucleotides as minimal binding site.
  • 基因功能參考文獻：
    1. Data suggest GS (glutamine synthetase) controls TnrA activity at positively controlled promoters by stabilizing TnrA in DNA-bound state; ATP and glutamine bind to GS in competitive manner; AMP competes with ATP and favors GS--TnrA interaction. PMID: 26635369
    2. TnrA inhibits the biosynthetic activity of glutamine synthetase PMID: 23535029
    3. TnrA binding to GlnK or glutamine synthetase responds differentially to adenylate nucleotide levels, with ATP weakening interactions with both partners. PMID: 21435182
    4. The negative control of the expression of the ilv-leu operon by TnrA, which is considered to represent rather fine-tuning (two- to threefold), is a novel regulatory link between nitrogen and amino acid metabolism. PMID: 15547269
    5. The biochemical properties of NrgB and its interactions with NrgA and TnrA are reported. PMID: 17001076
    6. TnrA transcription factor regulates gene expression during nitrogen-limited growth. PMID: 17085574
    7. The proteolytic degradation of TnrA as a response to nitrogen starvation represent a novel mechanism of TnrA control in B. subtilis. PMID: 18667567

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  • 亞細胞定位：
    Cell membrane.
  • 數據庫鏈接：


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