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  4. Recombinant Bacillus subtilis GTP-sensing transcriptional pleiotropic repressor CodY (codY)

Recombinant Bacillus subtilis GTP-sensing transcriptional pleiotropic repressor CodY (codY)

  • 中文名稱：
    Recombinant Bacillus subtilis GTP-sensing transcriptional pleiotropic repressor CodY(codY)
  • 貨號：
    CSB-YP331075BRJ
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Bacillus subtilis GTP-sensing transcriptional pleiotropic repressor CodY(codY)
  • 貨號：
    CSB-EP331075BRJ
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Bacillus subtilis GTP-sensing transcriptional pleiotropic repressor CodY(codY)
  • 貨號：
    CSB-EP331075BRJ-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Bacillus subtilis GTP-sensing transcriptional pleiotropic repressor CodY(codY)
  • 貨號：
    CSB-BP331075BRJ
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Bacillus subtilis GTP-sensing transcriptional pleiotropic repressor CodY(codY)
  • 貨號：
    CSB-MP331075BRJ
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    codY
  • Uniprot No.：
  • 別名：
    codY; BSU16170GTP-sensing transcriptional pleiotropic repressor CodY; Vegetative protein 286B; VEG286B
  • 種屬：
    Bacillus subtilis (strain 168)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    2-259
  • 氨基酸序列
    ALLQKTRII NSMLQAAAGK PVNFKEMAET LRDVIDSNIF VVSRRGKLLG YSINQQIEND RMKKMLEDRQ FPEEYTKNLF NVPETSSNLD INSEYTAFPV ENRDLFQAGL TTIVPIIGGG ERLGTLILSR LQDQFNDDDL ILAEYGATVV GMEILREKAE EIEEEARSKA VVQMAISSLS YSELEAIEHI FEELDGNEGL LVASKIADRV GITRSVIVNA LRKLESAGVI ESRSLGMKGT YIKVLNNKFL IELENLKSH
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    DNA-binding protein that represses the expression of many genes that are induced as cells make the transition from rapid exponential growth to stationary phase and sporulation. It is a GTP-binding protein that senses the intracellular GTP concentration as an indicator of nutritional limitations. At low GTP concentration it no longer binds GTP and stop to act as a transcriptional repressor.
  • 基因功能參考文獻：
    1. a comparison between wild-type B. subtilis and codY null mutant cells failed to reveal a role for CodY in regulating the braB gene, coding for a branched-chain amino acid permease PMID: 27744611
    2. The increase in BraB expression only at intermediate activities of CodY may facilitate the uptake of BCAA when they are not in excess but prevent unneeded BraB synthesis when other BCAA transporters are active PMID: 26473603
    3. Guanine derivative, most likely GTP, controls CodY activity. PMID: 21856856
    4. Changes in the rate of endogenous isoleucine, leucine and valine (ILV) synthesis serve as an important signal for CodY-mediated gene regulation. PMID: 20935095
    5. contols IlvB operon transcription PMID: 15916605
    6. involved in transcription regulation of ilv-leu operon together with CcpA and TnrA regulators of cellular catabolic and anabolic genes PMID: 15916606
    7. The role of a helix-turn-helix motif of codY in DNA binding is reported. PMID: 15937175
    8. Transcription of the acetate kinase gene (ackA) proved to be activated a global regulatory protein, CodY. PMID: 16995897
    9. Interaction of CodY with GTP is specific and results in increased affinity for its target genes. PMID: 17993518
    10. Results show that versions of the AATTTTCWGAAAATT motif, first identified for Lactococcus lactis CodY, with up to five mismatches play an important role in the interaction of B. subtilis CodY with DNA. PMID: 18083814
    11. Binding studies with branched-chain amino acids and their analogs revealed that an amino group at position 2 and a methyl group at position 3 of valine are critical components of the recognition of the amino acids by CodY. PMID: 19749041
    12. Results indicate that the transition state regulator ScoC, together with CodY and AbrB, negatively regulates bacilysin production in B. subtilis. [ScoC] PMID: 19801406

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  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    CodY family
  • 數據庫鏈接：


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