Recombinant Bacillus subtilis D-alanine--D-alanyl carrier protein ligase (dltA)

中文名稱：

枯草芽孢桿菌dltA重組蛋白
貨號：

CSB-YP336594BRJ(A4)
規格：
來源：

Yeast
其他：

中文名稱：

枯草芽孢桿菌dltA重組蛋白
貨號：

CSB-EP336594BRJ(A4)-B
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

枯草芽孢桿菌dltA重組蛋白
貨號：

CSB-BP336594BRJ(A4)
規格：
來源：

Baculovirus
其他：

中文名稱：

枯草芽孢桿菌dltA重組蛋白
貨號：

CSB-MP336594BRJ(A4)
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

dltA
Uniprot No.：

P39581
別名：

dltA; dae; BSU38500; ipa-5rD-alanine--D-alanyl carrier protein ligase; DCL; EC 6.2.1.54; D-alanine--poly(phosphoribitol) ligase subunit 1; D-alanine-activating enzyme; DAE
種屬：

Bacillus subtilis (strain 168)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Catalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D-alanyl carrier protein (Dcp) DltC. In an ATP-dependent two-step reaction, forms a high energy D-alanyl-AMP intermediate, followed by transfer of the D-alanyl residue as a thiol ester to the phosphopantheinyl prosthetic group of the Dcp. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.
基因功能參考文獻：
1. DltA synthesizes not only N-(d-alanyl)-l-cysteine (a dipeptide) but also various oligopeptides. PMID: 26586916
2. assayed the substrate selectivity of DltA with its substrate DltC PMID: 15955059
3. analysis of the crystal structure of DltA PMID: 18784082
亞細胞定位：

Cytoplasm.
蛋白家族：

ATP-dependent AMP-binding enzyme family, DltA subfamily
數據庫鏈接：

KEGG: bsu:BSU38500

STRING: 224308.Bsubs1_010100020786