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  4. Recombinant Bacillus subtilis ATP-dependent helicase/deoxyribonuclease subunit B (addB), partial

Recombinant Bacillus subtilis ATP-dependent helicase/deoxyribonuclease subunit B (addB), partial

  • 中文名稱：
    Recombinant Bacillus subtilis ATP-dependent helicase/deoxyribonuclease subunit B(addB) ,partial
  • 貨號：
    CSB-YP328410BRJ
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Bacillus subtilis ATP-dependent helicase/deoxyribonuclease subunit B(addB) ,partial
  • 貨號：
    CSB-EP328410BRJ
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Bacillus subtilis ATP-dependent helicase/deoxyribonuclease subunit B(addB) ,partial
  • 貨號：
    CSB-EP328410BRJ-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Bacillus subtilis ATP-dependent helicase/deoxyribonuclease subunit B(addB) ,partial
  • 貨號：
    CSB-BP328410BRJ
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Bacillus subtilis ATP-dependent helicase/deoxyribonuclease subunit B(addB) ,partial
  • 貨號：
    CSB-MP328410BRJ
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    addB
  • Uniprot No.：
  • 別名：
    addB; BSU10620; ATP-dependent helicase/deoxyribonuclease subunit B; EC 3.1.-.-; EC 3.6.4.12; ATP-dependent helicase/nuclease AddB
  • 種屬：
    Bacillus subtilis (strain 168)
  • 蛋白長度：
    Partial
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    The heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent single-stranded exonuclease, acting in both directions. Recognizes the B.subtilis chi site (5'-AGCGG-3') which transforms the enzyme from a helicase which degrades both DNA strands to one with only 5' to 3' exonuclease activity. This generates a double-stranded DNA with a protruding 3'-terminated single-stranded tail suitable for the initiation of homologous recombination (chi fragment). The AddB nuclease domain is not required for chi fragment generation; this subunit has 5' -> 3' nuclease activity. RecA thread formation during DNA double-strand break repair requires RecJ or AddAB.
  • 基因功能參考文獻(xiàn)：
    1. Chi hotspots trigger a conformational change in the helicase-like domain of AddAB to activate homologous recombination. PMID: 26762979
    2. transient binding of AddAB to chi(Bs) is an integral part of the AddAB-chi(Bs) interaction and propose that this molecular event underlies a general mechanism for regulating the biochemical activities and biological functions of RecBCD-like enzymes PMID: 16632468
    3. Results show that the AddAB enzyme complex contains two active nuclease domains, each of which is dedicated to cleavage of one specific DNA strand. PMID: 17570399
  • 蛋白家族：
    Helicase family, AddB/RexB type 1 subfamily
  • 數(shù)據(jù)庫鏈接：


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