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  4. Recombinant Bacillus phage phi29 DNA terminal protein (3)

Recombinant Bacillus phage phi29 DNA terminal protein (3)

  • 中文名稱：
    Recombinant Bacillus phage phi29 DNA terminal protein(3)
  • 貨號(hào)：
    CSB-YP366016BBC
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Bacillus phage phi29 DNA terminal protein(3)
  • 貨號(hào)：
    CSB-EP366016BBC
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Bacillus phage phi29 DNA terminal protein(3)
  • 貨號(hào)：
    CSB-EP366016BBC-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Bacillus phage phi29 DNA terminal protein(3)
  • 貨號(hào)：
    CSB-BP366016BBC
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Bacillus phage phi29 DNA terminal protein(3)
  • 貨號(hào)：
    CSB-MP366016BBC
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    3
  • Uniprot No.：
  • 別名：
    3Primer terminal protein; TP; DNA terminal protein; Gene product 3; gp3; Protein p3; Terminal protein
  • 種屬：
    Bacillus phage phi29 (Bacteriophage phi-29)
  • 蛋白長度：
    full length protein
  • 表達(dá)區(qū)域：
    1-266
  • 氨基酸序列
    MARSPRIRIK DNDKAEYARL VKNTKAKIAR TKKKYGVDLT AEIDIPDLDS FETRAQFNKW KEQASSFTNR ANMRYQFEKN AYGVVASKAK IAEIERNTKE VQRLVDEKIK AMKDKEYYAG GKPQGTIEQR IAMTSPAHVT GINRPHDFDF SKVRSYSRLR TLEESMEMRT DPQYYEKKMI QLQLNFIKSV EGSFNSFDAA DELIEELKKI PPDDFYELFL RISEISFEEF DSEGNTVENV EGNVYKILSY LEQYRRGDFD LSLKGF
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Acts as a primer for DNA elongation during viral genomic replication. Acts as the small terminase protein during packaging. Recruits the phage DNA polymerase to the bacterial nucleoid. Primer terminal protein (TP) is covalently linked to the 5'-ends of both strands of the genome through a phosphodiester bond between the beta-hydroxyl group of a serine residue and the 5'-phosphate of the terminal deoxyadenylate (dAMP). To start replication, the DNA polymerase forms a heterodimer with a free TP that recognizes the replication origins at both 5' ends of the linear chromosome, and initiates replication using as primer the OH-group of Ser-232 of the TP. Since the polymerase initiates the replication on the second thymine, the TP-dAMP initiation product slides backwards to recover the template information of the first nucleotide.; Hydrolyzes host peptidoglycans during virus entry.
  • 基因功能參考文獻(xiàn)：
    1. Data show that the terminal protein (TP) N-terminal mutants affected in DNA binding are impaired in protein-primed initiation of varphi29 TP-DNA replication. PMID: 25722367
  • 亞細(xì)胞定位：
    Virion. Host nucleus.
  • 蛋白家族：
    Phi29likevirus DNA terminal protein family
  • 數(shù)據(jù)庫鏈接：

    KEGG: vg:6446514



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