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  4. Recombinant B-box type zinc finger protein ncl-1 (ncl-1), partial

Recombinant B-box type zinc finger protein ncl-1 (ncl-1), partial

  • 中文名稱：
    秀麗隱桿線蟲ncl-1重組蛋白
  • 貨號：
    CSB-YP327347CXY
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲ncl-1重組蛋白
  • 貨號：
    CSB-EP327347CXY
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲ncl-1重組蛋白
  • 貨號：
    CSB-EP327347CXY-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲ncl-1重組蛋白
  • 貨號：
    CSB-BP327347CXY
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    秀麗隱桿線蟲ncl-1重組蛋白
  • 貨號：
    CSB-MP327347CXY
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ncl-1
  • Uniprot No.：
  • 別名：
    ncl-1; ZK112.2; B-box type zinc finger protein ncl-1
  • 種屬：
    Caenorhabditis elegans
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Translational repressor that inhibits protein synthesis. Represses the translation of mRNAs such as fib-1, probably by being recruited by RNA-binding protein nos-2 and the Pumilio proteins puf-5, puf-8 and puf-9 to the consensus core PUF binding motif in the 3'-UTR of fib-1 mRNA. Negatively regulates ribosomal RNA (rRNA) synthesis, ribosomal protein synthesis and nucleolus size. Its role in the negative regulation of nucleolus size is most likely through its negative regulation of the translation of proteins such as the rRNA 2'-O-methyltransferase fib-1, and dao-5. Might act directly as a transcription factor to inhibit RNA polymerase I (rRNA) and III (5S RNA) transcription. Plays a role in embryonic development, and in particular, is involved in regulating the localization of proteins, such as par-2, that are required for embryonic cell polarity. Plays a role in the regulation of lifespan, and the response to nutrient availability.
  • 基因功能參考文獻：
    1. NCL-1 extends lifespan and limits nucleolar size in the major C. elegans longevity pathways, as part of a convergent mechanism focused on the nucleolus. PMID: 28853436
    2. NCL-1 suppressed the translation of FIB-1/fibrillarin, while let-7 targeted the 3'UTR of ncl-1 and inhibited its expression. Consequently, the abundance of FIB-1 is tightly controlled and correlated with the nucleolar size PMID: 26492166
  • 亞細胞定位：
    Cytoplasm.
  • 組織特異性：
    Present in cells in which nucleoli are absent, and absent from large cells in which nucleoli are prominent. Highly expressed in the gonads.
  • 數據庫鏈接：

    STRING: 6239.ZK112.2

    UniGene: Cel.17103



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