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  4. Recombinant Aspergillus oryzae Adenylyltransferase and sulfurtransferase uba4 (uba4)

Recombinant Aspergillus oryzae Adenylyltransferase and sulfurtransferase uba4 (uba4)

  • 中文名稱：
    米曲霉uba4重組蛋白
  • 貨號：
    CSB-YP647216DPA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    米曲霉uba4重組蛋白
  • 貨號：
    CSB-EP647216DPA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    米曲霉uba4重組蛋白
  • 貨號：
    CSB-EP647216DPA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    米曲霉uba4重組蛋白
  • 貨號：
    CSB-BP647216DPA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    米曲霉uba4重組蛋白
  • 貨號：
    CSB-MP647216DPA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    uba4
  • Uniprot No.：
  • 別名：
    uba4; cnxF; AO090010000489Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4) [Includes: Molybdopterin-synthase adenylyltransferase; EC 2.7.7.80; Adenylyltransferase uba4; Sulfur carrier protein MOCS2A adenylyltransferase); Molybdopterin-synthase sulfurtransferase; EC 2.8.1.11; Sulfur carrier protein MOCS2A sulfurtransferase; Sulfurtransferase uba4)]
  • 種屬：
    Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-463
  • 氨基酸序列
    MARTVRDAAW VLGAIAGRDG RDNYTLASPH PISENTHRVT HERIIPDVTR SSQLKLRSAK VLIVGAGGLG CPAAQYLAGA GVGTLGLVDG DTVESSNLHR QVLHRSKNVG KLKVDSAIES LRDLNPHPTY IAHRAHLAPQ DAADIFKNYD LILDCTDNPA TRYLISDTAV LLGKPLVSAS ALRTEGQLMV LNNPPRSVGD KTGGPCYRCV FPRPPPANSI MSCADGGILG PVVGTMGVLQ ALEAIKVITA TDEEVKPPSL HIFSAYSSPL FRTIKLRSRR PNCAVCSGEA SVTLETVKSG STDYVFFCGS VGPEKLLLPE ERISPREYRT KYPVTASSEA IGTVTKEPTI IDVREKVQFD ICSLENSINI PISSILSSAT KTAQNNEVAN GSNPLPPWLP ADIASSDSID PIYVVCRLGN DSQIAVKRLK ELGLDRGGER VVADIRGGLR AWREQVDPEW PEY
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers urm1 and mocs2a. Its N-terminus first activates urm1 and mocs2a as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to urm1 and mocs2a to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards urm1 and mocs2a. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor; nfs1 probably acting as a sulfur donor for thiocarboxylation reactions.
  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    HesA/MoeB/ThiF family, UBA4 subfamily
  • 數據庫鏈接：


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