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  4. Recombinant Arabidopsis thaliana Zinc finger protein CONSTANS (CO)

Recombinant Arabidopsis thaliana Zinc finger protein CONSTANS (CO)

  • 中文名稱：
    Recombinant Arabidopsis thaliana Zinc finger protein CONSTANS(CO)
  • 貨號：
    CSB-YP658575DOA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Zinc finger protein CONSTANS(CO)
  • 貨號：
    CSB-EP658575DOA
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Zinc finger protein CONSTANS(CO)
  • 貨號：
    CSB-EP658575DOA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Zinc finger protein CONSTANS(CO)
  • 貨號：
    CSB-BP658575DOA
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Zinc finger protein CONSTANS(CO)
  • 貨號：
    CSB-MP658575DOA
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    CO
  • Uniprot No.：
  • 別名：
    CO; At5g15840; F14F8_220Zinc finger protein CONSTANS
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-373
  • 氨基酸序列
    MLKQESNDIG SGENNRARPC DTCRSNACTV YCHADSAYLC MSCDAQVHSA NRVASRHKRV RVCESCERAP AAFLCEADDA SLCTACDSEV HSANPLARRH QRVPILPISG NSFSSMTTTH HQSEKTMTDP EKRLVVDQEE GEEGDKDAKE VASWLFPNSD KNNNNQNNGL LFSDEYLNLV DYNSSMDYKF TGEYSQHQQN CSVPQTSYGG DRVVPLKLEE SRGHQCHNQQ NFQFNIKYGS SGTHYNDNGS INHNAYISSM ETGVVPESTA CVTTASHPRT PKGTVEQQPD PASQMITVTQ LSPMDREARV LRYREKRKTR KFEKTIRYAS RKAYAEIRPR VNGRFAKREI EAEEQGFNTM LMYNTGYGIV PSF
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Transcription factor that acts in the long day flowering pathway and may mediate between the circadian clock and the control of flowering. Plays a role in the regulation of flowering time by acting on 'SUPPRESSOR OF OVEREXPRESSION OF CO1', 'TERMINAL FLOWER 1' and 'FLOWERING LOCUS T'. Also regulates P5CS2 and ACS10 (involved in proline and ethylene biosynthesis, respectively). Regulates the expression of NAKR1 by binding to the 5'-TGTG(N2-3)ATG-3' motif.
  • 基因功能參考文獻：
    1. The clock-controlled mechanism by which photoperiodic information regulates CONSTANS function is the key mechanism in Arabidopsis. PMID: 27688622
    2. A heavy-metal-associated (HMA) domain-containing protein, SODIUM POTASSIUM ROOT DEFECTIVE 1 (NaKR1), is activated by CONSTANS (CO) under long-day conditions and regulates long-distance movement of FT in Arabidopsis. Loss of function of NaKR1 compromises FT transport to shoot apices through sieve elements, causing late flowering under long-day conditions. PMID: 27255839
    3. CO expression during night time temperatures and flowering PMID: 26856528
    4. Data indicate that CONSTANS (CO) plays an active role in sustaining its diurnal accumulation dynamics during Arabidopsis photoperiodic flowering. PMID: 27607358
    5. Results indicate that the DELLA-CONSTANS (CO) cascade inhibits CO/FLOWERING LOCUS T (FT)-mediated flowering under LDs, which thus provide evidence to directly integrate gibberellin (GA) and photoperiod signaling to synergistically modulate flowering under long days (LDs). PMID: 27406167
    6. PRR-mediated stabilization increases binding of CO to the promoter of FLOWERING LOCUS T (FT), leading to enhanced FT transcription and early flowering under these conditions. PMID: 28270524
    7. CO protein phosphorylation contributes to the photoperiodic flowering response by enhancing the rate of CO turnover via activity of the COP1 ubiquitin ligase. PMID: 26358558
    8. Interaction of CO with miP1a/b/TPL causes late flowering due to a failure in the induction of FLOWERING LOCUS T (FT) expression under inductive long day conditions. PMID: 27015278
    9. these findings suggest that regulation of flowering by gibberellin (GA) signaling in leaves under long days is mediated through repression of DELLA proteins on CO, providing a molecular link between DELLA proteins, key components in GA signaling pathway, and CO, a critical flowering activator in photoperiod signaling pathway. PMID: 26801684
    10. Red light-mediated degradation of CO protein by HOS1 ligase regulates photoperiodic flowering in Arabidopsis. PMID: 26373454
    11. BBX19 as a circadian clock output that depletes the active CO pool to accurately monitor daylength and precisely time FT expression. PMID: 25228341
    12. Natural variation in the cis-regulatory sequence in CONSTANS underlies flowering time diversity in Arabidopsis. PMID: 24736505
    13. report that TARGET OF EAT1 (TOE1) and related proteins interact with the activation domain of CONSTANS (CO) and CO-like (COL) proteins and inhibit CO activity. PMID: 25934507
    14. PFT1 is an activator of CO transcription, and also of FT transcription, in a CO-independent manner. PFT1 acts as a hub, integrating a variety of interdependent environmental stimuli, including light quality and jasmonic acid-dependent defences. PMID: 21985558
    15. Photoperiodic flowering regulators CO and GI are involved in FT- and TSF-mediated stomatal opening induced by blue light. PMID: 23669744
    16. study demonstrates that FKF1 protein stabilizes CONSTANS (CO)protein in the afternoon in long days; together with CO transcriptional regulation, FKF1 protein controls robust FLOWERING LOCUS T (FT) mRNA induction through multiple feedforward mechanisms that accurately control flowering timing PMID: 22628657
    17. Overexpression of all FBH genes drastically elevated CO levels and caused early flowering regardless of photoperiod, whereas CO levels were reduced in the fbh quadruple mutants. PMID: 22334645
    18. CONSTANS was found to bind DNA via a unique sequence element containing a consensus TGTG(N2-3)ATG motif present in tandem within the FLOWERING LOCUS T promoter. PMID: 20406410
    19. CONSTANS is repressed by the E3 ligase DAY NEUTRAL FLOWERING, which prevents early flowering in short days. PMID: 20435904
    20. CO-derived signal(s), or possibly CO itself, fits the definition of the hypothetical flowering stimulant, florigen. PMID: 15299137
    21. findings show that FKF1 controls daily CONSTANS expression in part by degrading CYCLING DOF FACTOR 1 (CDF1), a repressor of CONSTANS transcription PMID: 16002617
    22. Promotes flowering specifically under long days. PMID: 16006578
    23. RFI2 represses the expression of CONSTANS. PMID: 16709197
    24. These data suggest that CO might replace At HAP2 in the HAP complex to form a trimeric CO/At HAP3/At HAP5 complex. PMID: 17138697
    25. COb was retained for a long period after duplication, but a recent fixation of a detrimental mutation, possibly as an effect of a bottleneck, resulted in its nonfunctionalization in brassica nigra. PMID: 17344804
    26. determines flowering timing with circadian clock. PMID: 18453150
    27. a positive regulator of floral induction, as an OBF4-interacting protein PMID: 18587275
    28. regulation of CO by light quality likely plays a key role in the regulation of flowering time in natural environments PMID: 18667727
    29. A quantitative balance between the activator CO and the repressor TEMPRANILLO genes determines Flowering locus T levels. PMID: 18718758
    30. Data suggest that antagonism between GIGANTEA and DOF1/2 transcription factors contributes to photoperiodic flowering by modulating an underlying diurnal rhythm in CONSTANS transcript levels. PMID: 19619493

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    CONSTANS family
  • 組織特異性：
    Expressed in leaves, shoots and shoot apical meristem. Detected in the vascular tissue of the hypocotyl, the cotyledons and the leaves. Restricted to the protoxylem and phloem in young inflorescence stems and to the phloem only in older inflorescences. Al
  • 數據庫鏈接：

    KEGG: ath:AT5G15840

    STRING: 3702.AT5G15840.1

    UniGene: At.18



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