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  4. Recombinant Arabidopsis thaliana Vacuolar protein sorting-associated protein 35A (VPS35A), partial

Recombinant Arabidopsis thaliana Vacuolar protein sorting-associated protein 35A (VPS35A), partial

  • 中文名稱：
    擬南芥VPS35A重組蛋白
  • 貨號：
    CSB-YP800012DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥VPS35A重組蛋白
  • 貨號：
    CSB-EP800012DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥VPS35A重組蛋白
  • 貨號：
    CSB-EP800012DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥VPS35A重組蛋白
  • 貨號：
    CSB-BP800012DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥VPS35A重組蛋白
  • 貨號：
    CSB-MP800012DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    VPS35A
  • Uniprot No.：
  • 別名：
    VPS35A; ZIP3; At2g17790; T17A5.7; Vacuolar protein sorting-associated protein 35A; Protein ZIG SUPPRESSOR 3; Vesicle protein sorting 35A
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Plays a role in vesicular protein sorting. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. Also involved in the efficient sorting of seed storage proteins. Binds alone to endosomal membranes and is required for recruitment of VPS26 and VPS29 to membrane. The VPS29-VPS26-VPS35 subcomplex may be involved in recycling of specific cargos from endosome to the plasma membrane.
  • 基因功能參考文獻：
    1. show that, out of the three VPS35 retromer subunits present in Arabidopsis thaliana genome, the VPS35 homolog A plays a prevailing role in trafficking to the lytic vacuole PMID: 23770835
    2. Arabidopsis Rab7 homolog RABG3f participates in the recruitment of the core retromer to the endosomal membrane by interacting with VPS35 PMID: 23362252
    3. zip3 is a loss-of-function mutant of VPS35A. Expression of VPS35B or VPS35C cannot complement the function of VPS35A. PMID: 20086190
    4. findings suggest that VPS35, mainly VPS35b, is involved in sorting proteins to PSVs in seeds, possibly by recycling VSR from PVCs to the Golgi complex, and is also involved in plant growth and senescence in vegetative organs PMID: 18222962
  • 亞細胞定位：
    Cytoplasm. Endosome membrane; Peripheral membrane protein; Cytoplasmic side. Prevacuolar compartment membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    VPS35 family
  • 數據庫鏈接：

    KEGG: ath:AT2G17790

    STRING: 3702.AT2G17790.1

    UniGene: At.40097



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