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  4. Recombinant Arabidopsis thaliana Shaggy-related protein kinase eta (ASK7)

Recombinant Arabidopsis thaliana Shaggy-related protein kinase eta (ASK7)

  • 中文名稱：
    Recombinant Arabidopsis thaliana Shaggy-related protein kinase eta(ASK7)
  • 貨號：
    CSB-YP655093DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Shaggy-related protein kinase eta(ASK7)
  • 貨號：
    CSB-EP655093DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Shaggy-related protein kinase eta(ASK7)
  • 貨號：
    CSB-EP655093DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Shaggy-related protein kinase eta(ASK7)
  • 貨號：
    CSB-BP655093DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    Recombinant Arabidopsis thaliana Shaggy-related protein kinase eta(ASK7)
  • 貨號：
    CSB-MP655093DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    ASK7
  • Uniprot No.：
  • 別名：
    ASK7; BIN2; DWF12; SK21; UCU1; At4g18710; F28A21.120Shaggy-related protein kinase eta; EC 2.7.11.1; ASK-eta; Protein BRASSINOSTEROID INSENSITIVE 2; Protein ULTRACURVATA 1; Shaggy-related protein kinase 21; AtSK21
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-380
  • 氨基酸序列
    MADDKEMPAA VVDGHDQVTG HIISTTIGGK NGEPKQTISY MAERVVGTGS FGIVFQAKCL ETGETVAIKK VLQDRRYKNR ELQLMRVMDH PNVVCLKHCF FSTTSKDELF LNLVMEYVPE SLYRVLKHYS SANQRMPLVY VKLYMYQIFR GLAYIHNVAG VCHRDLKPQN LLVDPLTHQV KICDFGSAKQ LVKGEANISY ICSRFYRAPE LIFGATEYTT SIDIWSAGCV LAELLLGQPL FPGENAVDQL VEIIKVLGTP TREEIRCMNP HYTDFRFPQI KAHPWHKIFH KRMPPEAIDF ASRLLQYSPS LRCTALEACA HPFFDELREP NARLPNGRPF PPLFNFKQEV AGSSPELVNK LIPDHIKRQL GLSFLNQSGT
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Negative regulator in brassinosteroid signal transduction pathway important for plant growth. May be also involved in auxin signaling pathway. Phosphorylates and increases the degradation of BZR1 and BZR2/BES1 by the proteasome. Phosphorylates BHLH150, beet curly top virus C4 and tomato golden mosaic virus AC4 on threonine and serine residues. Upon brassinosteroid signaling, inhibits stomatal development by phosphorylating and inhibiting the MAPKK kinase YDA and the MAPK kinases MKK4 and MKK5. Phosphorylates BSK1, BSK3, BSK5, BSK6, BSK8 AND BSK11 in vitro. Phoyphorylates and destabilizes WRKY46, WRKY54 and WRKY70. Mediates BASL nuclear exclusion; kinase activity is required for this function. Required first at the cortical polarity site, to restrict MAPK signaling and promote asymmetric cell division (ACD), and second in the nucleus of stomatal lineage ground cells (SLGCs) or meristemoids, to limit cell division and to promote differentiation into pavement or stomatal guard cells, respectively, likely by initiating BASL polarization. Phosphorylates BASL, YDA and SPCH in vitro and POLAR in vivo. Phosphorylates and inhibits SPCH in the nucleus of SLGC undergoing ACD, thus negatively regulating stomatal development.
  • 基因功能參考文獻：
    1. COP1/SPA complex promotes PIF3 stability by repressing BIN2 activity. PMID: 28292892
    2. Point mutations of the BIN2-mediated CESA1 phosphorylation site abolished BIN2-dependent regulation of cellulose synthase activity. PMID: 28289192
    3. TOR-S6K2-BIN2 signaling pathway plays important roles in regulating the photoautotrophic growth of Arabidopsis. PMID: 27479935
    4. The hda6 mutant showed a BR-repressed phenotype in the dark and was less sensitive to BR biosynthesis inhibitors. Genetic analysis indicated that HDA6 regulates BR signaling through BIN2. Furthermore, we identified K189 of BIN2 as an acetylated site, which can be deacetylated by HDA6 to influence BIN2 activity PMID: 27562168
    5. KIB1 plays an essential role in brassinosteroid signaling by inhibiting BIN2 through dual mechanisms of blocking substrate access and promoting degradation. PMID: 28575660
    6. BIN2 phosphorylates HAT1 and probably stabilizes HAT1. PMID: 24164091
    7. phosphorylation of ARF7 and ARF19 by BRASSINOSTEROID-insensitive2 (BIN2) can also potentiate auxin signalling output during lateral root organogenesis. PMID: 24362628
    8. The results suggest that AGB1 interacts with BIN2, but regulates the BR signalling in a BZR1-independent manner. PMID: 23814276
    9. brassinosteroid inhibits stomatal development by alleviating GSK3-mediated inhibition of this MAPK module, providing two key links; that of a plant MAPKKK to its upstream regulators and of brassinosteroid to a specific developmental output PMID: 22307275
    10. BIN2 utilizes a direct kinase-substrate docking mechanism to phosphorylate BZR1 and regulate its protein stability PMID: 20522560
    11. Nuclear BIN2 induced phosphorylation and nuclear export of BES1 more efficiently than cytosolic BIN2. PMID: 20387035
    12. Proteasome-mediated protein degradation constitutes an important regulatory mechanism for restricting the BIN2 activity in Arabidopsis. PMID: 18726001
    13. BIN2 plays a dominant role among the three group II members in regulating brassinosteroid signaling. PMID: 19395409

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  • 亞細胞定位：
    Cytoplasm, cell cortex. Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, GSK-3 subfamily
  • 組織特異性：
    In the two outer cell layers of the developing seed coat and restricted to the suspensor cells in developing embryos. Mostly expressed in stomatal lineage cells with asymmetric cell division (ACD) potential. Observed in small cells of non-protruding hypoc
  • 數據庫鏈接：

    KEGG: ath:AT4G18710

    STRING: 3702.AT4G18710.1

    UniGene: At.22875



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