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  4. Recombinant Arabidopsis thaliana Serine/threonine-protein phosphatase PP1 isozyme 4 (TOPP4)

Recombinant Arabidopsis thaliana Serine/threonine-protein phosphatase PP1 isozyme 4 (TOPP4)

  • 中文名稱：
    擬南芥TOPP4重組蛋白
  • 貨號：
    CSB-YP341768DOA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥TOPP4重組蛋白
  • 貨號：
    CSB-EP341768DOA
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥TOPP4重組蛋白
  • 貨號：
    CSB-EP341768DOA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥TOPP4重組蛋白
  • 貨號：
    CSB-BP341768DOA
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥TOPP4重組蛋白
  • 貨號：
    CSB-MP341768DOA
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    TOPP4
  • Uniprot No.：
  • 別名：
    TOPP4; At2g39840; T5I7.14; Serine/threonine-protein phosphatase PP1 isozyme 4; EC 3.1.3.16; Type one protein phosphatase 4
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-321
  • 氨基酸序列
    MATTTTTQGQ QTAIDSAVLD DIIRRLTEVR LARPGKQVQL SEAEIKQLCT TARDIFLQQP NLLELEAPIK ICGDIHGQYS DLLRLFEYGG FPPSANYLFL GDYVDRGKQS LETICLLLAY KIKYPGNFFL LRGNHECASI NRIYGFYDEC KRRFNVRVWK VFTDCFNCLP VAALIDDKIL CMHGGLSPDL DHLDEIRNLP RPTMIPDTGL LCDLLWSDPG KDVKGWGMND RGVSYTFGPD KVSEFLTKHD LDLVCRAHQV VEDGYEFFAD RQLVTVFSAP NYCGEFDNAG AMMSVDENLM CSFQILKPAE KKTKFMMSTK I
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Serine/threonine-protein phosphatase that possesses phosphatase activity toward para-nitrophenyl phosphate (pNPP) in vitro. Acts as positive regulator in the gibberellin (GA) signaling pathway to regulate plant growth and development. Promotes the GA-induced and proteasomal-dependent degradation of the DELLA proteins RGA and GAI by directly binding and dephosphorylating these proteins. Involved in the regulation of phytochrome B (phyB) signaling pathway that controls photomorphogenesis. Promotes the proteasomal-dependent degradation of PIF5 factor by directly binding and dephosphorylating this protein. Involved in the regulation of pavement cell (PC) interdigitation by modulating the auxin efflux carrier PIN1 polarity and endocytic trafficking. Regulates PIN1 polar targeting through direct binding and dephosphorylation. Acts antagonistically with PID in regulating PC development.
  • 基因功能參考文獻：
    1. outline a novel phytochrome signaling mechanism by which TOPP4-mediated dephosphorylation of PIF5 attenuates phytochrome-dependent light responses PMID: 26704640
    2. TOPP4-regulated PIN1 polar targeting through direct dephosphorylation is crucial for pavement cell morphogenesis in the Arabidopsis leaf. PMID: 25560878
    3. These studies provide direct evidence for a crucial role of protein dephosphorylation mediated by TOPP4 in the GA signaling pathway. PMID: 25010794
  • 亞細胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    PPP phosphatase family, PP-1 subfamily
  • 組織特異性：
    Expressed in the vasculature of roots and cotyledons, tips of leaves, guard cells, bases of trichomes, pistils and stamen filaments.
  • 數據庫鏈接：

    KEGG: ath:AT2G39840

    STRING: 3702.AT2G39840.1

    UniGene: At.310



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