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  4. Recombinant Arabidopsis thaliana Respiratory burst oxidase homolog protein D (RBOHD), partial

Recombinant Arabidopsis thaliana Respiratory burst oxidase homolog protein D (RBOHD), partial

  • 中文名稱：
    擬南芥RBOHD重組蛋白
  • 貨號：
    CSB-YP887766DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥RBOHD重組蛋白
  • 貨號：
    CSB-EP887766DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥RBOHD重組蛋白
  • 貨號：
    CSB-EP887766DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥RBOHD重組蛋白
  • 貨號：
    CSB-BP887766DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥RBOHD重組蛋白
  • 貨號：
    CSB-MP887766DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RBOHD
  • Uniprot No.：
  • 別名：
    RBOHD; At5g47910; MCA23.25; Respiratory burst oxidase homolog protein D; EC 1.11.1.-; EC 1.6.3.-; NADPH oxidase RBOHD; AtRBOHD
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Calcium-dependent NADPH oxidase that generates superoxide. Involved in the generation of reactive oxygen species (ROS) during incompatible interactions with pathogens and in UV-B and abscisic acid ROS-dependent signaling. Might be required for ROS signal amplification during light stress.
  • 基因功能參考文獻：
    1. Differential recovery between the accessions was related to the activity of three genes: RESPIRATORY BURST OXIDASE HOMOLOG D, SENESCENCE-ASSOCIATED GENE113, and ORESARA1, which function in a regulatory network involving a reactive oxygen species (ROS) burst upon desubmergence and the hormones abscisic acid and ethylene. PMID: 29891679
    2. induction of stress marker genes and reactive oxygen species (ROS)-scavenging enzyme genes under various stress conditions is dependent on the ERF74-RbohD-ROS signal pathway. PMID: 28164334
    3. The role of NADPH oxidases under cadmium (Cd) toxicity was studied using Arabidopsis thaliana mutants AtrbohC, AtrbohD and AtrbohF. PMID: 26765289
    4. RBOHD role in the systemic response to salt stress PMID: 27261066
    5. differential spatio-temporal expression of the Rboh genes contributes to fine-tune RBOH/NADPH oxidase-dependent reactive oxygen species production and signaling in Arabidopsis immunity. PMID: 26798024
    6. The involvement of hydrogen peroxide (H2O2) generated by nicotinamide adenine dinucleotide phosphate-oxidase (NADPH oxidase) in the antioxidant defense system was assessed in salt-challenged Arabidopsis thaliana seedlings. PMID: 25462961
    7. Systemic acquired acclimation in plants is stress specific and that the reactive oxygen species wave is associated with the production of systemic variation potentials, specifically linking the electric signals with RBOHD function in A. thaliana. PMID: 24038652
    8. infection of Arabidopsis thaliana by the parasitic nematode Heterodera schachtii activated the NADPH oxidases RbohD and RbohF to produce ROS, which was necessary to restrict infected plant cell death and promote nurse cell formation. PMID: 24714570
    9. These findings suggest that both AtrbohD and AtrbohF are essential for abscissic acid-promoted reactive oxygen species production in roots. PMID: 23963673
    10. Mutation of Cys 890 compromised S-nitrosothiol-mediated control of AtRBOHD activity, perturbing the magnitude of cell death development. This cysteine is evolutionarily conserved and specifically S-nitrosylated in both human and fly NADPH oxidase, suggesting that this mechanism may govern immune responses in both plants and animals. [RBOHD] PMID: 21964330
    11. Rhobd plays a role in the process of stomatal closure in response to salicylic acid. PMID: 23500715
    12. There is no additive effect of disease symptoms or bacterial growth in the AtrbohD x Atgox1 and AtrbohD x Atgox3 double mutants when compared with individual mutants. PMID: 22751316
    13. Data suggest that specific sphingobases can induce cell death and require respiratory burst oxidase-D (RBOHD) for early reactive oxygen species induction in plants. PMID: 21856300
    14. Mild salt stress triggers a rapid increase in RbohD expression in Arabidopsis. PMID: 21205037
    15. Reactive oxygen species generated by Atrbohs are closely related to the jasmonic acid signaling pathway. PMID: 21421415
    16. RBOHD triggers death in cells that are damaged by fungal infection but simultaneously inhibits death in neighboring cells through the suppression of free salicylic acid and ethylene levels. PMID: 19726575
    17. Ca(2+) binding and phosphorylation synergistically activate the ROS-producing enzyme activity of AtrbohD. PMID: 18218618
    18. The rbohD mutant expressing non-PA-binding RbohD was compromised in ABA-mediated ROS production and stomatal closure. PMID: 19690149
    19. Data show that a rapid systemic signal that traveled at a rate of 8.4 centimeters per minute and was dependent on the respiratory burst oxidase homolog D (RbohD) gene. PMID: 19690331

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  • 亞細胞定位：
    Membrane; Multi-pass membrane protein.
  • 蛋白家族：
    RBOH (TC 5.B.1.3) family
  • 組織特異性：
    More abundant in roots than in leaves, stems or inflorescences. Expressed in mesophyll and guard cells.
  • 數據庫鏈接：

    KEGG: ath:AT5G47910

    STRING: 3702.AT5G47910.1

    UniGene: At.23270



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