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  4. Recombinant Arabidopsis thaliana RAN GTPase-activating protein 1 (RANGAP1), partial

Recombinant Arabidopsis thaliana RAN GTPase-activating protein 1 (RANGAP1), partial

  • 中文名稱：
    擬南芥RANGAP1重組蛋白
  • 貨號：
    CSB-YP888707DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥RANGAP1重組蛋白
  • 貨號：
    CSB-EP888707DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥RANGAP1重組蛋白
  • 貨號：
    CSB-EP888707DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥RANGAP1重組蛋白
  • 貨號：
    CSB-BP888707DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥RANGAP1重組蛋白
  • 貨號：
    CSB-MP888707DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    RANGAP1
  • Uniprot No.：
  • 別名：
    RANGAP1; At3g63130; T20O10.230; RAN GTPase-activating protein 1; AtRanGAP1; RanGAP1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state. Plays a role in spatial signaling during cell division.
  • 基因功能參考文獻：
    1. The data show that plant development is differentially affected by RanGAP mutant allele combinations of increasing severity and requires the GAP activity of RanGAP, while the subcellular positioning of RanGAP is dispensable. PMID: 26091693
    2. These results show that the two RanGAPs are redundant and indispensable for female gametophyte development in Arabidopsis but dispensable for pollen development. PMID: 21282324
    3. AtRanGAP1 has a mitotic trafficking pattern uniquely different from that of vertebrate RanGAP. PMID: 15807788
    4. In a wip1-1/wip2-1/wip3-1 triple mutant, RanGAP1 is dislocated from the nuclear envelope in undifferentiated root-tip cells, whereas nuclear envelope targeting in differentiated root cells and targeting to the cell plate remain intact. PMID: 17600715
    5. Data suggest that an unanticipated complexity of RanGAP nuclear envelope targeting and at least one member of each NE-associated coiled-coil and transmembrane domains protein family is required for RanGAP targeting in root tip cells. PMID: 18591351
    6. propose that Arabidopsis RanGAP, a continuous positive protein marker of the plant division plane, has a role in spatial signaling during plant cell division PMID: 19011093
    7. These data suggest that both HSC70-1 and the WPP-domain proteins play a role in facilitating WIT1 nuclear envelope targeting; this may be the first described in planta activity for the WPP-domain proteins. PMID: 19617588

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  • 亞細胞定位：
    Cytoplasm. Nucleus envelope. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, phragmoplast. Note=Localized in patchy areas at the nuclear envelope (NE) of interphase cells. Concentrates at the preprophase band (PPB) and remains associated with the cortical division site (CDS) during mitosis and cytokinesis. During mitosis, associates with mitotic spindles at the anaphase. Associated to the microtubular phragmoplast and the surface of the daughter nuclei at the telophase.
  • 蛋白家族：
    RNA1 family
  • 數據庫鏈接：

    KEGG: ath:AT3G63130

    STRING: 3702.AT3G63130.1

    UniGene: At.48804



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