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  4. Recombinant Arabidopsis thaliana Protein phosphatase 2C 16 (HAB1)

Recombinant Arabidopsis thaliana Protein phosphatase 2C 16 (HAB1)

  • 中文名稱：
    擬南芥HAB1重組蛋白
  • 貨號：
    CSB-YP861273DOA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥HAB1重組蛋白
  • 貨號：
    CSB-EP861273DOA
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥HAB1重組蛋白
  • 貨號：
    CSB-EP861273DOA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥HAB1重組蛋白
  • 貨號：
    CSB-BP861273DOA
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥HAB1重組蛋白
  • 貨號：
    CSB-MP861273DOA
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    HAB1
  • Uniprot No.：
  • 別名：
    HAB1; P2C-HA; At1g72770; F28P22.4; Protein phosphatase 2C 16; AtPP2C16; EC 3.1.3.16; AtP2C-HA; Protein HYPERSENSITIVE TO ABA 1; Protein phosphatase 2C HAB1; PP2C HAB1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Full Length of Mature Protein
  • 表達區域：
    23-511
  • 氨基酸序列
    SPVEITQL KNVTDAADLL SDSENQSFCN GGTECTMEDV SELEEVGEQD LLKTLSDTRS GSSNVFDEDD VLSVVEDNSA VISEGLLVVD AGSELSLSNT AMEIDNGRVL ATAIIVGESS IEQVPTAEVL IAGVNQDTNT SEVVIRLPDE NSNHLVKGRS VYELDCIPLW GTVSIQGNRS EMEDAFAVSP HFLKLPIKML MGDHEGMSPS LTHLTGHFFG VYDGHGGHKV ADYCRDRLHF ALAEEIERIK DELCKRNTGE GRQVQWDKVF TSCFLTVDGE IEGKIGRAVV GSSDKVLEAV ASETVGSTAV VALVCSSHIV VSNCGDSRAV LFRGKEAMPL SVDHKPDRED EYARIENAGG KVIQWQGARV FGVLAMSRSI GDRYLKPYVI PEPEVTFMPR SREDECLILA SDGLWDVMNN QEVCEIARRR ILMWHKKNGA PPLAERGKGI DPACQAAADY LSMLALQKGS KDNISIIVID LKAQRKFKTR T
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Key component and repressor of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, seed germination and inhibition of vegetative growth. Confers enhanced sensitivity to drought.
  • 基因功能參考文獻：
    1. HAB1.1 and HAB1.2, that play opposing roles in abscisic acid -mediated seed germination and ABA-mediated post-germination. developmental arrest. PMID: 26419884
    2. We have identified HAB1 C186 and C274 as H2O2-sensitive thiols and demonstrate that their oxidation inhibits both HAB1 catalytic activity and its ability to physically associate with SnRK2.6 by formation of intermolecular dimers. PMID: 25460914
    3. Data demonstrated that HAB1 dephosphorylates and deactivates OST1 in vitro. HAB1 and the related PP2Cs ABI1 and ABI2 interact with OST1 in vivo, and mutations in the corresponding genes strongly affect OST1 activation by ABA. PMID: 19855047
    4. PYL5 binds ABA with micromolar or nanomolar affinity in the absence or presence of HAB1, respectively. PMID: 19624469
    5. show that plants expressing a mutant form of HAB1 in which Gly246 was mutated to Asp (G246D) display strong ABA insensitive phenotypes PMID: 16876791
    6. HAB1 modulates the abscisic acid response by regulation SWI3B. PMID: 19033529
    7. findings show that abscissic binds to PYRABACTIN RESISTANCE 1 (PYR1), which in turn binds to and inhibits Type 2C protein phosphatases (HAB1) PMID: 19407142

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  • 亞細胞定位：
    Cytoplasm. Nucleus. Note=Mainly cytoplasmic.
  • 蛋白家族：
    PP2C family
  • 組織特異性：
    Expressed in seeds, roots, stems, leaves and flowers, especially in meristematic tissues, guard cells, embryo and siliques.
  • 數據庫鏈接：

    KEGG: ath:AT1G72770

    STRING: 3702.AT1G72770.1

    UniGene: At.46635



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