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  4. Recombinant Arabidopsis thaliana Protein TRANSPORT INHIBITOR RESPONSE 1 (TIR1), partial

Recombinant Arabidopsis thaliana Protein TRANSPORT INHIBITOR RESPONSE 1 (TIR1), partial

  • 中文名稱：
    擬南芥TIR1重組蛋白
  • 貨號：
    CSB-YP710089DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥TIR1重組蛋白
  • 貨號：
    CSB-EP710089DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥TIR1重組蛋白
  • 貨號：
    CSB-EP710089DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥TIR1重組蛋白
  • 貨號：
    CSB-BP710089DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥TIR1重組蛋白
  • 貨號：
    CSB-MP710089DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 別名：
    TIR1; FBL1; WEI1; At3g62980; T20O10.80; Protein TRANSPORT INHIBITOR RESPONSE 1; Weak ethylene-insensitive protein 1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder Warning: in_array() expects parameter 2 to be array, null given in /www/web/cusabio_cn/public_html/caches/caches_template/default/content/show_product_protein.php on line 662
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Auxin receptor that mediates Aux/IAA proteins proteasomal degradation and auxin-regulated transcription. The SCF(TIR1) E3 ubiquitin ligase complex is involved in auxin-mediated signaling pathway that regulate root and hypocotyl growth, lateral root formation, cell elongation, and gravitropism. Appears to allow pericycle cells to overcome G2 arrest prior to lateral root development. Plays a role in ethylene signaling in roots. Confers sensitivity to the virulent bacterial pathogen P.syringae.
  • 基因功能參考文獻：
    1. In vitro and in vivo protein-protein interaction assays show that NO enhances ASK1 binding to CUL1 and TIR1/AFB2, required for SCF(TIR1/AFB2) assembly. PMID: 30031268
    2. The miR393-TIR1 molecular regulation pathway appears to be a component of the regulatory control over shoot regeneration from in vitro culture. PMID: 29241559
    3. TIR1/AFB-Aux/IAA auxin perception mediates rapid cell wall acidification and growth of Arabidopsis hypocotyls. PMID: 27627746
    4. HSP90 stabilizes auxin receptor TIR1 and ensures plasticity of auxin responses PMID: 28532230
    5. We propose oligomerization of TIR1 as a novel regulatory mechanism in the regulation of auxin-mediated plant patterning and development. PMID: 27618443
    6. These results demonstrate that HSP90 masks a point mutation in the auxin receptor TIR1 and thereby buffers auxin-responsive phenotypes. PMID: 27816945
    7. This study showed show that increased temperature promotes rapid accumulation of the TIR1 auxin co-receptor, an effect that is dependent on the molecular chaperone HSP90, and that HSP90 and SGT1 integrate temperature and auxin signaling. PMID: 26728313
    8. Overexpression of a miR393-resistant TIR1 gene (mTIR1) in Arabidopsis clearly enhanced salt stress tolerance. PMID: 25336111
    9. Auxin-binding protein 1 is a negative regulator of the SCF(TIR1/AFB) pathway. PMID: 24051655
    10. Changes in the leucine-rich repeat domain of the TIR1 auxin coreceptor can alter the properties of SCF(TIR1). PMID: 23539280
    11. This effect of elevated auxin levels requires the activity of SKP-Cullin-F-box(TIR1/AFB) (SCF(TIR1/AFB))-dependent pathway. Importantly, also suboptimal auxin levels mediate PIN degradation utilizing the same signalling pathway. PMID: 23211744
    12. Mutations of ASA1 and the TIR1/AFBs auxin receptor genes impair the inhibitory effect of 5 muM MeJA on PIN2 endocytosis and enhance the reduction effect of 50 muM MeJA on the plasma membrane accumulation of PIN2. PMID: 21466556
    13. TIR1 auxin receptor family is not involved in auxin-induced H(+)-ATPase phosphorylation. PMID: 22492846
    14. miR393 and secondary siRNAs regulate expression of the TIR1 PMID: 21828251
    15. The interaction between miR393 and its target indicates a fine adjustment to the roles of the miR393-TIR1 module, which is required for auxin responses in plant development. PMID: 22042293
    16. the TIR1/AFB proteins have distinct biochemical activities, and TIR1 and AFB2 are the dominant auxin receptors in the seedling root PMID: 20018756
    17. Biochemical studies show that IAA1/AXR5 interacts with TIR1 in an auxin-dependent manner. PMID: 15546359
    18. results indicate that TIR1 is an auxin receptor that mediates Aux/IAA degradation and auxin-regulated transcription PMID: 15917797
    19. TIR1 is an auxin receptor mediating transcriptional responses to auxin PMID: 15917798
    20. crystal structures of the Arabidopsis TIR1-ASK1 complex, free and in complexes with three different auxin compounds and an Aux/IAA substrate peptide PMID: 17410169
    21. Modulation of auxin sensitivity by phosphate (Pi) availability was found to depend on the auxin receptor TRANSPORT INHIBITOR RESPONSE1 (TIR1) and the transcription factor AUXIN RESPONSE FACTOR19 (ARF19). PMID: 19106375
    22. COP9 signalosome- and 26S proteasome-dependent regulation of SCFTIR1 accumulation in Arabidopsis PMID: 19147500
    23. Auxin-enhanced TIR1-mediated ubiquitination of SHY2 and IAA12. PMID: 19309453

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  • 亞細胞定位：
    Nucleus.
  • 組織特異性：
    Expressed in roots, stems, leaves and flowers. In adult plants, mostly expressed in floral stigma, anther filaments, abscission zones and vascular tissues.
  • 數據庫鏈接：

    KEGG: ath:AT3G62980

    STRING: 3702.AT3G62980.1

    UniGene: At.25594



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