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  4. Recombinant Arabidopsis thaliana Protein TOC75-3, chloroplastic (TOC75-3), partial

Recombinant Arabidopsis thaliana Protein TOC75-3, chloroplastic (TOC75-3), partial

  • 中文名稱：
    擬南芥TOC75-3重組蛋白
  • 貨號：
    CSB-YP871154DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥TOC75-3重組蛋白
  • 貨號：
    CSB-EP871154DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥TOC75-3重組蛋白
  • 貨號：
    CSB-EP871154DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥TOC75-3重組蛋白
  • 貨號：
    CSB-BP871154DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥TOC75-3重組蛋白
  • 貨號：
    CSB-MP871154DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    TOC75-3
  • Uniprot No.：
  • 別名：
    TOC75-3; TOC75; At3g46740; T6H20.230; Protein TOC75-3; chloroplastic; 75 kDa translocon at the outer-envelope-membrane of chloroplasts 3; AtTOC75-III
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Essential protein. Mediates the insertion of proteins targeted to the outer membrane of chloroplasts. Required for the import of protein precursors into chloroplasts. Forms the voltage-dependent preprotein translocation channels (hydrophilic beta barrel) of the TOC complex in the chloroplastic outer membrane.
  • 基因功能參考文獻：
    1. Toc75 polypeptide transport-associated (POTRA) domains are located in the intermembrane space of the chloroplast envelope by immunogold electron microscopy. PMID: 27388682
    2. Data indicate that OEP80-T7(atToc75-V) and atToc75-III showed distinct migration patterns on blue native-PAGE. PMID: 22094888
    3. the POTRA domains of both Toc75-III and Toc75-V are exposed to the cytoplasm PMID: 21825140
    4. SPase I converted Toc75 precursor to its mature form in vitro. PMID: 16275749
    5. Embryo development in the atToc75-III mutant, lacking the pore forming component of the translocase, was arrested extremely early at the two-cell stage. PMID: 16435266
    6. atTOC75-III of the Toc75 (translocon at the outer envelope membrane of chloroplasts, 75 kD) gene family is essential for plant viability, and is expressed at its highest level in young, rapidly expanding tissues PMID: 15908591

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  • 亞細胞定位：
    Plastid, chloroplast outer membrane; Multi-pass membrane protein.
  • 蛋白家族：
    TOC75 family
  • 組織特異性：
    Mostly expressed in young and actively dividing photosynthetic tissues and, to a lower extent, in old leaves and roots. Particularly low levels in leaves after etiolation.
  • 數據庫鏈接：

    KEGG: ath:AT3G46740

    STRING: 3702.AT3G46740.1

    UniGene: At.3633



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