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  4. Recombinant Arabidopsis thaliana Protein SCARECROW (SCR), partial

Recombinant Arabidopsis thaliana Protein SCARECROW (SCR), partial

  • 中文名稱：
    擬南芥SCR重組蛋白
  • 貨號：
    CSB-YP881909DOA
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥SCR重組蛋白
  • 貨號：
    CSB-EP881909DOA
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥SCR重組蛋白
  • 貨號：
    CSB-EP881909DOA-B
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥SCR重組蛋白
  • 貨號：
    CSB-BP881909DOA
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥SCR重組蛋白
  • 貨號：
    CSB-MP881909DOA
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    SCR
  • Uniprot No.：
  • 別名：
    SCR; SGR1; At3g54220; F24B22.180; Protein SCARECROW; AtSCR; GRAS family protein 20; AtGRAS-20; Protein SHOOT GRAVITROPISM 1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Transcription factor required for quiescent center cells specification and maintenance of surrounding stem cells, and for the asymmetric cell division involved in radial pattern formation in roots. Essential for cell division but not differentiation of the ground tissue. Also required for normal shoot gravitropism. Regulates the radial organization of the shoot axial organs. Binds to the promoter of MGP, NUC, RLK and SCL3. Restricts SHR movment and sequesters it into the nucleus of the endodermis.
  • 基因功能參考文獻：
    1. This study showed that INDETERMINATE DOMAIN PROTEIN (ID) binding sequences have a crucial role in the regulation of SCARECROW and SHORT-ROOT expression.[SCARECROW] PMID: 28324206
    2. In the crystal structures of the SHR-SCR binary and JACKDAW (JKD)/IDD10-SHR-SCR ternary complexes, each GRAS domain comprises one alpha/beta core subdomain with an alpha-helical cap that mediates heterodimerization by forming an intermolecular helix bundle. PMID: 28211915
    3. SCR and PLETHORA genetically and physically interact with plant-specific teosinte-branched cycloidea PCNA (TCP) transcription factors to specify the stem cell niche during embryogenesis and maintain organizer cells post-embryonically. PMID: 30018102
    4. results provide new insights into the regulatory role of the SHR-SCR-SCL23 network in the endodermis development in both roots and shoots. PMID: 27353361
    5. QC precursor cells originated from the outer layer of stage II lateral root primordia, within which the SCARECROW (SCR) transcription factor was specifically expressed. Disrupting SCR function abolished periclinal divisions in this lateral root primordia cell layer and perturbed the formation of QC precursor cells. PMID: 27510971
    6. This study showed that INDETERMINATE DOMAIN PROTEIN binding sequences have a crucial role in the regulation of SCARECROW and SHORT-ROOT expression. PMID: 28324206
    7. modeling, transcriptional reporters, and synthetic promoters support a mechanism whereby expression at the top of the SHORTROOT-SCARECROW cascade is established through opposing activities of activators and repressors PMID: 27923776
    8. SCARECROW controls Arabidopsis root meristem size from the root endodermis tissue by regulating the DELLA protein RGA that in turn mediates the regulation of ARR1 levels at the transition zone. PMID: 26848984
    9. Data indicate that SEUSS (SEU) gene has distinct genetic interactions with SHORT-ROOT (SHR), SCARECROW (SCR), and SCARECROW-LIKE3 (SCL3) genes. PMID: 26818732
    10. Data show that SCARECROW-LIKE23 (SCL23) is a mobile protein that controls movement of SHORT-ROOT (SHR) and acts redundantly with SCARCROW (SCR) to specify endodermal fate in the root meristem. PMID: 26415082
    11. Mutations in three GRAS family transcription factors, SHORT-ROOT (SHR), SCARECROW (SCR) and SCARECROW-LIKE 23 (SCL23), affect BS cell fate in Arabidopsis thaliana. PMID: 24517883
    12. SCARECROW reinforces SHORT-ROOT signaling and inhibits periclinal cell divisions in the ground tissue by maintaining SHR at high levels in the endodermis. PMID: 23072993
    13. A defect in SGR1 leads to leaf stay-green phenotypes in Arabidopsis and rice. PMID: 24043799
    14. SHRUBBY (At5g24740) controls root growth downstream of gibberellic acid in part through the regulation of SHORT-ROOT and SCARECROW. PMID: 23444357
    15. SHR and SCR regulate a similar but not identical set of stress response genes. PMID: 22312006
    16. JKD directly regulates SCR and MGP expression in cooperation with SHR, SCR and MGP. PMID: 21935722
    17. SHR and SCR primarily function as general regulators of cell proliferation in leaves. PMID: 20739610
    18. findings show that SCARECROW (SCR) blocks SHORTROOT (SHR) movement by sequestering it into the nucleus through protein-protein interaction and a safeguard mechanism that relies on a SHR/SCR-dependent positive feedback loop for SCR transcription PMID: 17446396
    19. LHP1 acts together with SCR to suppress premature middle cortex formation. PMID: 19228333

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  • 亞細胞定位：
    Nucleus.
  • 蛋白家族：
    GRAS family
  • 組織特異性：
    Expressed in siliques, leaves and roots. Detected in the initial daughter cell before its asymmetric division and remains expressed only in the endodermal cell layer after the division. Expressed in the endodermis or starch sheath of the seedling hypocoty
  • 數據庫鏈接：

    KEGG: ath:AT3G54220

    STRING: 3702.AT3G54220.1

    UniGene: At.71814



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