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  4. Recombinant Arabidopsis thaliana Probable serine/threonine-protein kinase GCN2 (GCN2), partial

Recombinant Arabidopsis thaliana Probable serine/threonine-protein kinase GCN2 (GCN2), partial

  • 中文名稱：
    擬南芥GCN2重組蛋白
  • 貨號：
    CSB-YP862916DOA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥GCN2重組蛋白
  • 貨號：
    CSB-EP862916DOA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥GCN2重組蛋白
  • 貨號：
    CSB-EP862916DOA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥GCN2重組蛋白
  • 貨號：
    CSB-BP862916DOA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥GCN2重組蛋白
  • 貨號：
    CSB-MP862916DOA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    GCN2
  • Uniprot No.：
  • 別名：
    GCN2; At3g59410; F25L23_270; eIF-2-alpha kinase GCN2; serine/threonine-protein kinase GCN2; EC 2.7.11.1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評價

靶點詳情

  • 功能：
    Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 eIF-2-alpha in response to low amino acid availability. Plays a role as an activator of the general amino acid control pathway required for adapatation to amino acid starvation. Converts phosphorylated eIF-2-alpha either to a competitive inhibitor of translation initiation, leading to a global protein synthesis repression, and thus to a reduced overall utilization of amino acids, or to a translational initiation activation of specific mRNAs, and hence allowing reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Binds uncharged tRNAs.
  • 基因功能參考文獻：
    1. In concert with GCN2, TOR allows photo-autotrophic eukaryotes to coordinate the fluxes of carbon, nitrogen, and sulfur for efficient cysteine biosynthesis under varying external nutrient supply PMID: 29079776
    2. Although both ILA and GCN2 genes are necessary to mediate eIF2alpha phosphorylation upon treatments with the aromatic amino acid biosynthesis inhibitor glyphosate, their mutants develop distinct root and chloroplast phenotypes. PMID: 29680783
    3. These results points to an unknown GCN2-dependent factor involved in the cascade of events triggered by glyphosate in plants. PMID: 25603772
    4. AtGCN2 plays pivotal roles in various cellular processes essential for normal growth and development. PMID: 25912940
    5. Analysis of wild-type and GT8359 (null mutant for AtGCN2) plants infected with Turnip yellow mosaic virus or Turnip crinkle virus showed that AteIF2alpha was not phosphorylated. PMID: 18603615
    6. GCN2 is activated following wounding and exposure to hormones. GCN2 can phosphorylate eIF2alpha and is essential in amino acid deprivation conditions. PMID: 19108716

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  • 亞細胞定位：
    Cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family, GCN2 subfamily
  • 組織特異性：
    Expressed in roots, leaves, stems, buds, flowers, siliques and seedlings.
  • 數(shù)據(jù)庫鏈接：

    KEGG: ath:AT3G59410

    STRING: 3702.AT3G59410.2

    UniGene: At.34633



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