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  4. Recombinant Arabidopsis thaliana Probable lysine-specific demethylase JMJ14 (JMJ14), partial

Recombinant Arabidopsis thaliana Probable lysine-specific demethylase JMJ14 (JMJ14), partial

  • 中文名稱：
    擬南芥JMJ14重組蛋白
  • 貨號：
    CSB-YP818105DOA
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥JMJ14重組蛋白
  • 貨號：
    CSB-EP818105DOA
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥JMJ14重組蛋白
  • 貨號：
    CSB-EP818105DOA-B
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥JMJ14重組蛋白
  • 貨號：
    CSB-BP818105DOA
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥JMJ14重組蛋白
  • 貨號：
    CSB-MP818105DOA
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    JMJ14
  • Uniprot No.：
  • 別名：
    JMJ14; JMJ4; PKDM7B; At4g20400; F9F13.50Probable lysine-specific demethylase JMJ14; EC 1.14.11.-; Jumonji domain-containing protein 14; Jumonji domain-containing protein 4; Lysine-specific histone demethylase JMJ14; Protein JUMONJI 14
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Transcriptional repressor. Histone demethylase that demethylates 'Lys-4' (H3K4me) of histone H3 with a higher activity for H3K4me3 and H3K4me2 than H3K4me1. No activity on H3K9me3/2, H3K36me3/2 and H3K27me3/2. Represses FT and TSF expression to inhibit the floral transition. Binds around the transcription start site of the FT locus. Involved in the DRM2-mediated maintenance of DNA methylation, but not required for the de novo DNA methylation. Required for demethylating histone H3K4me3 at the target of RNA silencing. Together with NAC051/NAC052 and NAC050, regulates gene expression and flowering time, probably by the promotion of RNA-mediated gene silencing.
  • 基因功能參考文獻：
    1. Plants defective in JMJ14 exhibit reduced Histone3 Lysine9 Lysine14 acetylation (H3K9K14Ac) levels, reduced polymerase II (Pol II) occupancy, and reduced transgene transcription. PMID: 23001035
    2. JMJ14 is specifically involved in the maintenance phase of DRM2-mediated RNA-directed DNA methylation. [Jmj14] PMID: 21052090
    3. JMJ14 acts downstream from the Argonaute effector complex to demethylate histone H3K4 at the target of RNA silencing. [JMJ14] PMID: 20478993
    4. AtJmj4 and ELF6 are H3K4 demethylases directly repressing FT chromatin and preventing precocious flowering in Arabidopsis. [AtJmj4] PMID: 19946624
    5. PKDM7B is involved in H3K4 demethylation in FT and TSF chromatin, and represses expression of these genes to inhibit the floral transition. PMID: 20202164
    6. JMJ14 (At4g20400), is an active histone H3K4 demethylase and is involved in flowering time regulation. PMID: 20177424

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  • 亞細胞定位：
    Nucleus, nucleoplasm. Nucleus. Note=Not detected in the nucleolus and the chromocenters.
  • 蛋白家族：
    JARID1 histone demethylase family
  • 組織特異性：
    Expressed in shoot apex, primary root tip, trichomes of young leaves, leaf vascular tissues, anther filaments and styles. Detected in inflorescences, leaves, stems, roots and siliques. Mostly expressed in floral organs, and, at low levels, in other organs
  • 數(shù)據(jù)庫鏈接：

    KEGG: ath:AT4G20400

    STRING: 3702.AT4G20400.1

    UniGene: At.43482



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