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  4. Recombinant Arabidopsis thaliana Phospholipase D delta (PLDDELTA), partial

Recombinant Arabidopsis thaliana Phospholipase D delta (PLDDELTA), partial

  • 中文名稱：
    擬南芥PLDDELTA重組蛋白
  • 貨號：
    CSB-YP880190DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥PLDDELTA重組蛋白
  • 貨號：
    CSB-EP880190DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥PLDDELTA重組蛋白
  • 貨號：
    CSB-EP880190DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥PLDDELTA重組蛋白
  • 貨號：
    CSB-BP880190DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥PLDDELTA重組蛋白
  • 貨號：
    CSB-MP880190DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    PLDDELTA
  • Uniprot No.：
  • 別名：
    PLDDELTA; At4g35790; F4B14.60; Phospholipase D delta; AtPLDdelta; PLD delta; EC 3.1.4.4
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond to generate phosphatidic acids (PA). May be involved in PA accumulation in the dehydration stress response and in the transduction of hormonal and environmental signals to the microtubules cytoskeleton. Prefers phosphatidylethanolamine to phosphatidylcholine as substrate. Involved in H(2)O(2) and abscisic acid (ABA)-induced stomatal closure. Involved in nitric oxide (NO) signaling during stomatal closure. Plays a positive role in ABA-promoted senescence. Involved in basal defense and nonhost resistance.
  • 基因功能參考文獻：
    1. PLDdelta negatively regulates plant thermotolerance via destabilizing cortical microtubules. PMID: 28710795
    2. in wt plants PLDdelta disrupt membranes in severe drought stress and, in the absence of the protein (PLDdelta knock-out) might drought-prime the plants, making them more tolerant to severe drought stress. PMID: 26340512
    3. PLD is involved in defense signaling in nonhost resistance against powdery mildew fungi and PLDdelta is the main isoform participating in this process. PMID: 23979971
    4. PLDdelta-mediated hydrolysis of phosphatidylcholine to phosphatidic acid plays a positive role in abscisic acid-promoted senescence. PMID: 23762411
    5. Phospholipase Ddelta is downstream of nitric oxide and hydrogen peroxide in abscisic acid (ABA)-induced stomatal closure. PMID: 22932846
    6. Report show that the presence of PLDdelta correlates with reduced lipid hydrolysis during post-freezing recovery, suggesting a positive role for PLDdelta in freezing tolerance. PMID: 17962199

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  • 亞細胞定位：
    Cell membrane; Peripheral membrane protein.
  • 蛋白家族：
    Phospholipase D family, C2-PLD subfamily
  • 組織特異性：
    Expressed in roots, leaves, stems, siliques and flowers. Strongly expressed in the vascular tissues of cotyledons and leaves under dehydration stress conditions. Expression is higher in old leaves than in young leaves. Expressed in leaves and guard cells.
  • 數據庫鏈接：

    KEGG: ath:AT4G35790

    STRING: 3702.AT4G35790.1

    UniGene: At.22959



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