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  4. Recombinant Arabidopsis thaliana Phospholipase D alpha 1 (PLDALPHA1), partial

Recombinant Arabidopsis thaliana Phospholipase D alpha 1 (PLDALPHA1), partial

  • 中文名稱：
    擬南芥PLDALPHA1重組蛋白
  • 貨號：
    CSB-YP656435DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥PLDALPHA1重組蛋白
  • 貨號：
    CSB-EP656435DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥PLDALPHA1重組蛋白
  • 貨號：
    CSB-EP656435DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥PLDALPHA1重組蛋白
  • 貨號：
    CSB-BP656435DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥PLDALPHA1重組蛋白
  • 貨號：
    CSB-MP656435DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    PLDALPHA1
  • Uniprot No.：
  • 別名：
    PLDALPHA1; PLD1; At3g15730; MSJ11.13; Phospholipase D alpha 1; AtPLDalpha1; PLD alpha 1; EC 3.1.4.4; Choline phosphatase 1; PLDalpha; Phosphatidylcholine-hydrolyzing phospholipase D 1
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    Partial
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond to generate phosphatidic acids (PA). Plays an important role in various cellular processes, including phytohormone action and response to stress, characterized by acidification of the cell. Involved in wound induction of jasmonic acid. May be involved in membrane lipid remodeling. Probably involved in freezing tolerance by modulating the cold-responsive genes and accumulation of osmolytes. Can use phosphatidylcholine (PC), phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) as substrates, both in presence or in absence of PIP2. Its main substrate is phosphatidylcholine. Stimulates the intrinsic GTPase activity of GPA1 upon binding. Mediates the abscisic acid effects on stomata through interaction with GPA1 and the production of phosphatidic acid that bind to ABI1. Involved in seed aging and deterioration. Involved in microtubule stabilization and salt tolerance. Involved in abscisic acid-induced stomatal closure.
  • 基因功能參考文獻：
    1. PLDalpha1-mediated phosphatidic acid formation modulates desiccation sensitivity as applying its inhibitor improved seed desiccation tolerance and its suppression in protoplasts enhanced survival under dehydration. PMID: 28152567
    2. In Arabidopsis thaliana, PLDalpha1/PA might regulate cell development by modulating microtubule organization in an activity-dependent manner. PMID: 27864640
    3. The phospholipase Dalpha1 protein is a key component and modulator of the G-protein complex in affecting a subset of signaling pathways. PMID: 26935351
    4. PLDalpha1 and phosphatidic acid regulate microtubular organization and Ca(2+) increases during abscisic acid (ABA)-induced stomatal closing and that crosstalk among signaling lipid, Ca(2+), and microtubules is essential for ABA signaling. PMID: 24271006
    5. Ablation of guard cell-expressed copper amine oxidase gamma or phospholipase Dalpha1 gene retarded abscisic acid-induced H2O2 generation and stomatal closure. PMID: 24817219
    6. ACBP1 interacts with PLDalpha1 at the plasma membrane. Their interaction was further confirmed by yeast two-hybrid analysis. As recombinant ACBP1 binds phosphatidic acid and phosphatidylcholine, ACBP1 probably promotes PLDalpha1 action. PMID: 23448237
    7. PLDalpha1-derived phosphatidic acid (PA) binds to MAP65-1, thus mediating microtubule stabilization and salt tolerance. It reveals a functional connection between membrane lipids and the cytoskeleton in environmental stress signaling. PMID: 23150630
    8. PLDalpha1 and PLDdelta are involved in reactive oxygen species and nitric oxide production and cytosolic alkalization. PLDalpha1 and PLDdelta cooperate in ABA signaling in guard cells. PMID: 22392280
    9. SPHK/phyto-S1P and PLDalpha1A are co-dependent in amplification of response to ABA, mediating stomatal closure in Arabidopsis. PMID: 22275366
    10. Piriformospora indica-stimulated growth response is mediated by a pathway consisting of the PLD-PDK1-OXI1 cascade. PMID: 21625539
    11. PLDalpha1 mediates the abscisic acid effects on stomata through interaction with a protein phosphatase 2C & a heterotrimeric GTP-binding protein PMID: 16614222
    12. PLDalpha1 plays a key role in freezing tolerance of Arabidopsis by modulating the cold-responsive genes and accumulation of osmolytes. PMID: 16949955
    13. Identification of PLDalpha-mediated events in response to progressive drought stress in Arabidopsis is reported. PMID: 17261695
    14. A high level of PLDalpha1 is detrimental to seed quality, and attenuation of PLDalpha1 expression has the potential to improve oil stability, seed quality and seed longevity. PMID: 17565616
    15. Report show that the presence of PLDalpha1 correlates with phospholipid hydrolysis in both freezing and post-freezing phases, suggesting a negative role for PLDalpha1 in freezing tolerance.. PMID: 17962199
    16. AtPLDalpha1 is activated after wounding but is restricted to ruptured cells. PMID: 19220780
    17. The pldalpha1 mutant failed to produce ROS in guard cells in response to ABA. PMID: 19690149

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  • 亞細胞定位：
    Cytoplasm. Cell membrane; Peripheral membrane protein. Mitochondrion membrane. Microsome membrane. Vacuole. Cytoplasmic vesicle, clathrin-coated vesicle.
  • 蛋白家族：
    Phospholipase D family, C2-PLD subfamily
  • 組織特異性：
    Highly expressed in roots, stems and flowers, moderately in leaves, seedlings and siliques. Not detected in seeds.
  • 數據庫鏈接：

    KEGG: ath:AT3G15730

    STRING: 3702.AT3G15730.1

    UniGene: At.23882



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