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  4. Recombinant Arabidopsis thaliana Mitogen-activated protein kinase 4 (MPK4)

Recombinant Arabidopsis thaliana Mitogen-activated protein kinase 4 (MPK4)

  • 中文名稱：
    擬南芥MPK4重組蛋白
  • 貨號(hào)：
    CSB-YP013457DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥MPK4重組蛋白
  • 貨號(hào)：
    CSB-EP013457DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥MPK4重組蛋白
  • 貨號(hào)：
    CSB-EP013457DOA-B
  • 說明書：
  • 規(guī)格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥MPK4重組蛋白
  • 貨號(hào)：
    CSB-BP013457DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥MPK4重組蛋白
  • 貨號(hào)：
    CSB-MP013457DOA
  • 說明書：
  • 規(guī)格：
  • 來源：
    Mammalian cell
  • 其他：

產(chǎn)品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MPK4
  • Uniprot No.：
  • 別名：
    MPK4; At4g01370; F2N1.1; F2N1_2-t; Mitogen-activated protein kinase 4; AtMPK4; MAP kinase 4; EC 2.7.11.24
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長(zhǎng)度：
    full length protein
  • 表達(dá)區(qū)域：
    1-376
  • 氨基酸序列
    MSAESCFGSS GDQSSSKGVA THGGSYVQYN VYGNLFEVSR KYVPPLRPIG RGAYGIVCAA TNSETGEEVA IKKIGNAFDN IIDAKRTLRE IKLLKHMDHE NVIAVKDIIK PPQRENFNDV YIVYELMDTD LHQIIRSNQP LTDDHCRFFL YQLLRGLKYV HSANVLHRDL KPSNLLLNAN CDLKLGDFGL ARTKSETDFM TEYVVTRWYR APELLLNCSE YTAAIDIWSV GCILGETMTR EPLFPGKDYV HQLRLITELI GSPDDSSLGF LRSDNARRYV RQLPQYPRQN FAARFPNMSA GAVDLLEKML VFDPSRRITV DEALCHPYLA PLHDINEEPV CVRPFNFDFE QPTLTEENIK ELIYRETVKF NPQDSV
  • 蛋白標(biāo)簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產(chǎn)品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    The ANPs-MKK6-MPK4 module is involved in the regulation of plant cytokinesis during meiosis and mitosis. Essential to promote the progression of cytokinesis and for cellularization (formation of the cell plate) during male-specific meiosis. Involved in cortical microtubules organization and stabilization by regulating the phosphorylation state of microtubule-associated proteins such as MAP65-1. Involved in root hair development process. Negative regulator of systemic acquired resistance (SAR) and salicylic acid- (SA) mediated defense response. Required for jasmonic acid- (JA) mediated defense gene expression. May regulate activity of transcription factor controlling pathogenesis-related (PR) gene expression. Seems to act independently of the SAR regulatory protein NPR1 (Nonexpresser of PR1). Phosphorylates MKS1 and transcription factors WRKY25 and WRKY33. The MEKK1, MKK1/MKK2 and MPK4 function in a signaling pathway that modulates the expression of genes responding to biotic and abiotic stresses and also plays an important role in pathogen defense by negatively regulating innate immunity. Phosphorylates MEKK2 upon treatment with flg22. Involved in stomatal movement regulation by repressing HT1 and HT1-mediated GHR1 phosphorylation.
  • 基因功能參考文獻(xiàn)：
    1. H2S inhibited the opening of stomata under cold stress, which required the participation of MPK4. In conclusion, MPK4 is a downstream component of H2S-related cold-stress resistance, and H2S and MPK4 both regulated the cold response genes and stomatal movement to response the cold stress. PMID: 29024849
    2. AvrRpt2 specifically blocks the flagellin-induced activation of MPK4. PMID: 27208280
    3. Data show that MPK4 phosphorylation of MYB75 increases its stability and is essential for light-induced anthocyanin accumulation. Our findings reveal an important role for a MAPK pathway in light signal transduction. PMID: 27811015
    4. ASR3 functions as a transcriptional repressor regulated by microbe-associated molecular patterns-activated MPK4 to fine-tune plant immune gene expression. PMID: 25770109
    5. Mitogen-activated protein kinase 4 is a salicylic acid-independent regulator of growth but not of photosynthesis in Arabidopsis PMID: 24874867
    6. Arabidopsis MPK4 can interact with and be phosphorylated by the cytokinesis-related MAP kinase kinase, AtMKK6. PMID: 21575092
    7. MPK4 activity was found to compromise effector-triggered immunity conditioned by the Toll Interleukin-1 Receptor-nucleotide binding (NB)-Leu-rich repeat (LRR) receptors RPS4 and RPP4 but not by the coiled coil-NB-LRR receptors RPM1 and RPS2. PMID: 23115249
    8. MPK4 is shown to be involved in the regulation of mitosis/cytokinesis through modulation of the cell division plane and cytokinetic progression. PMID: 21155826
    9. These results indicate that MPK4 is the downstream target of MKK6/ANQ in the regulation of cytokinesis in Arabidopsis and that MPK11 is also involved in cytokinesis. PMID: 21098735
    10. Pseudomonas syringae effector protein AvrB interacts with Arabidopsis MPK4 and the HSP90 chaperone, and AvrB induces MPK4 activation in a manner promoted by HSP90 PMID: 20159621
    11. Treatment with elevated levels of sodium chloride inhibits the growth of mpk4 transgenic plants; the mekk1;mpk4 double-mutant combination causes seedling lethality. PMID: 17572407
    12. These data establish direct links between MPK4 and innate immunity and provide an example of how a plant MAP kinase can regulate gene expression by releasing transcription factors in the nucleus upon activation. PMID: 18650934

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  • 亞細(xì)胞定位：
    Cytoplasm. Nucleus. Cytoplasm, cytoskeleton.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
  • 組織特異性：
    Ubiquitous. Expressed in the veins and stomatal guard cells of leaf plates, petioles, stem, roots and flowers.
  • 數(shù)據(jù)庫(kù)鏈接：

    KEGG: ath:AT4G01370

    STRING: 3702.AT4G01370.1

    UniGene: At.19915



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