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  4. Recombinant Arabidopsis thaliana Methyl-CpG-binding domain-containing protein 7 (MBD7)

Recombinant Arabidopsis thaliana Methyl-CpG-binding domain-containing protein 7 (MBD7)

  • 中文名稱：
    擬南芥MBD7重組蛋白
  • 貨號：
    CSB-YP887775DOA
  • 說明書：
  • 規格：
  • 來源：
    Yeast
  • 其他：
  • 中文名稱：
    擬南芥MBD7重組蛋白
  • 貨號：
    CSB-EP887775DOA
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 其他：
  • 中文名稱：
    擬南芥MBD7重組蛋白
  • 貨號：
    CSB-EP887775DOA-B
  • 說明書：
  • 規格：
  • 來源：
    E.coli
  • 共軛：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名稱：
    擬南芥MBD7重組蛋白
  • 貨號：
    CSB-BP887775DOA
  • 說明書：
  • 規格：
  • 來源：
    Baculovirus
  • 其他：
  • 中文名稱：
    擬南芥MBD7重組蛋白
  • 貨號：
    CSB-MP887775DOA
  • 說明書：
  • 規格：
  • 來源：
    Mammalian cell
  • 其他：

產品詳情

  • 純度：
    >85% (SDS-PAGE)
  • 基因名：
    MBD7
  • Uniprot No.：
  • 別名：
    MBD7; At5g59800; MMN10.2; Methyl-CpG-binding domain-containing protein 7; AtMBD7; MBD07; Methyl-CpG-binding protein MBD7
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-306
  • 氨基酸序列
    MQTRSSSSPS ANHRRETQLQ IADPTSFCGK IMPGWTVVNR PRSSNNGVVD TYFIEPGTGR QFSSLEAIHR HLAGEVNDRR LTRAGSFFQD KTRVYEGSRT KQDHCGVEYA SKGFRLPRGW SVEEVPRKNS HYIDKYYVER KTGKRFRSLV SVERYLRESR NSIEQQLRVL QNRRGHSKDF RLPDGWIVEE KPRRSSSHID RSYIEPGTGN KFRSMAAVER YLISVGNITL DSVSMVHSER LPLLMNRNGI RFQSEVIDPN PPKKVKWVLT GSGGNMFTAN VRGSNVSSLV KHTWSEAFVS LIEDRS
  • 蛋白標簽：
    Tag?type?will?be?determined?during?the?manufacturing?process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 產品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 復溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Transcriptional regulator that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. May directly affect chromatin structure by inducing intra- and inter- chromatin compaction via bridging over multiple methylated CpG sites. Acts as an anti-silencing factor that prevents DNA hypermethylation and gene repression. Requires high mCG density for binding. Recognizes preferentially mCGs located in transposable elements. Required for active DNA demethylation. Prefers to target genomic loci around chromocenters.
  • 基因功能參考文獻：
    1. MBD7 binds to genomic regions with a high density of CG methylation.MBD7 physically associates with IDM3, and with IDM1 and IDM2 proteins.MBD7 and IDM3 prevent DNA hypermethylation and gene silencing. PMID: 25684209
    2. MBD7 is required for active DNA demethylation and antisilencing of the genomic loci with high densities of DNA methylation in Arabidopsis. PMID: 25593350
    3. The C-terminal, StkC motif plays an important role in fastening AtMBD7 to its chromosomal, CpG-methylated sites. PMID: 19647732
    4. The characterization of proteins interacting with MBD7, especially PRMT11, is reported. PMID: 17711414
    5. poly-MBD proteins can directly affect chromatin structure by inducing intra- and inter-chromatin compaction via bridging over multiple methylated CpG sites PMID: 18211904

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  • 亞細胞定位：
    Nucleus. Chromosome.
  • 組織特異性：
    Expressed in leaves, buds, flowers, stems, siliques, mature seeds and roots.
  • 數據庫鏈接：

    KEGG: ath:AT5G59800

    STRING: 3702.AT5G59800.1

    UniGene: At.29205



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